CAJ | 학술논문

[目的]应用SSR分子标记研究吉林省主推中晚熟期玉米品种的遗传多样性.[方法]通过10对引物对供试材料进行扩增,并用聚丙烯凝胶电泳进行检测.[结果]10对SSR引物在供试材料中共检测出110个等位基因变异,每对SSR引物榆测出的等位基因数为4～17个,平均每个位点11个,每个SSR位点的PIC变化范围在0.63～0.93之间,平均0.842.43个玉米杂交种之间的遗传相似系数变化范围为0.664～0.918,平均为0.774,其中样本间遗传相似系数在0.85以上的共有30个.结合聚类分析和各品种的谱系来源,结果表明43份吉林省主推中晚熟期玉米品种的遗传相似性相对较大.[结论]吉林省中晚熟期种质资源相对狭窄,有待于进一步引种创新.
[목적]응용SSR분자표기연구길림성주추중만숙기옥미품충적유전다양성.[방법]통과10대인물대공시재료진행확증,병용취병희응효전영진행검측.[결과]10대SSR인물재공시재료중공검측출110개등위기인변이,매대SSR인물유측출적등위기인수위4～17개,평균매개위점11개,매개SSR위점적PIC변화범위재0.63～0.93지간,평균0.842.43개옥미잡교충지간적유전상사계수변화범위위0.664～0.918,평균위0.774,기중양본간유전상사계수재0.85이상적공유30개.결합취류분석화각품충적보계래원,결과표명43빈길림성주추중만숙기옥미품충적유전상사성상대교대.[결론]길림성중만숙기충질자원상대협착,유대우진일보인충창신.
[Objective]The aim was to study the genetic diversity of dominant middle-late mature maize hybrids in Jilin Province by the use of Simple sequence repeats(SSRs)molecular mark.[Method]PCR amplification was carried out based on ten core SSR primers and the amplification products were detected through sodium dodecyl sulfate polyacrylamide gel electrophoresis.[Result]Ten core SSR primers gave clear and stable profiles amplified in samples of 43 maize hybrids.A total of 110 polymorphic amplified fragments were produced by 10 primers.The average number of allele per SSR locus was 11 which varied from 4 to 17.The polymorphism information content(P/C)for the SSR loci ranged from0.63 to 0.93 with an average of 0.842.Genetic similarities among the 43 maize hybrids varied from 0.664 to 0.918 with an average of 0.774and those among 30 maize hybrids were greater than 0.85.Combined with the cluster analysis and pedigree origin of 43 maize hybrids,it was found that there were more genetic similarities among the 43 maize hybrids.[Conclusion]The germplasm resources of middle-late mature maize hybrids in Jilin Province were comparatively narrow and waited for innovation.