中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2010年
4期
310-313
,共4页
孙璞%陈晓平%裴斐%马瑞霞%张燚%陈群%董维刚%陈维香%黄惠丽
孫璞%陳曉平%裴斐%馬瑞霞%張燚%陳群%董維剛%陳維香%黃惠麗
손박%진효평%배비%마서하%장일%진군%동유강%진유향%황혜려
乳头状瘤,内翻%乳头状瘤病毒感染%鼻疾病%增生%寡核苷酸序列分析
乳頭狀瘤,內翻%乳頭狀瘤病毒感染%鼻疾病%增生%寡覈苷痠序列分析
유두상류,내번%유두상류병독감염%비질병%증생%과핵감산서렬분석
Papilloma,inverted%Papillomavirns infections%Nose diseases%Hyperplasia%Oligonucleotide arry sequence analysis
目的 探讨人乳头状瘤病毒(human papillomavims,HPV)在鼻内翻性乳头状瘤(nasal inverted papilloma,NIP)中感染亚型的分布及其在病变发生、发展中的作用.方法 取101例NIP石蜡包埋组织标本(其中单纯NIP组77例,伴不典型增生NIP组22例,NIP癌变组2例),采用核酸分子快速导流杂交基因芯片分型技术(HybriMax)对21种HPV亚型(并区分高危亚型和低危亚型)同步进行检测和病毒分型,并以24例健康志愿者鼻腔黏膜组织为健康对照组.结果 NIP石蜡包埋组织标本HPV总阳性率为64.4%(65/101),对照组HPV均为阴性(0/24),两组比较差异有统计学意义(χ~2=32.178,P<0.05).单纯NIP组、伴不典型增生NIP组、NIP癌变组HPV阳性率分别为59.7%(46/77)、81.8%(18/22)和50.0%(1/2),单纯NIP组与伴不典型增生NIP组比较,HPV阳性率差异无统计学意义(χ~2=3.649,P=0.056).单纯NIP组与伴不典型增生NIP组的HPV高危亚型、低危亚型的构成比差异有统计学意义(χ~2=10.412,P<0.05).结论 NIP的发病与HPV感染密切相关,HPV高危亚型可能促使NIP向不典型增生发展.明确NIP中HPV亚型的分布情况对肿瘤临床行为的预测有一定指导意义.
目的 探討人乳頭狀瘤病毒(human papillomavims,HPV)在鼻內翻性乳頭狀瘤(nasal inverted papilloma,NIP)中感染亞型的分佈及其在病變髮生、髮展中的作用.方法 取101例NIP石蠟包埋組織標本(其中單純NIP組77例,伴不典型增生NIP組22例,NIP癌變組2例),採用覈痠分子快速導流雜交基因芯片分型技術(HybriMax)對21種HPV亞型(併區分高危亞型和低危亞型)同步進行檢測和病毒分型,併以24例健康誌願者鼻腔黏膜組織為健康對照組.結果 NIP石蠟包埋組織標本HPV總暘性率為64.4%(65/101),對照組HPV均為陰性(0/24),兩組比較差異有統計學意義(χ~2=32.178,P<0.05).單純NIP組、伴不典型增生NIP組、NIP癌變組HPV暘性率分彆為59.7%(46/77)、81.8%(18/22)和50.0%(1/2),單純NIP組與伴不典型增生NIP組比較,HPV暘性率差異無統計學意義(χ~2=3.649,P=0.056).單純NIP組與伴不典型增生NIP組的HPV高危亞型、低危亞型的構成比差異有統計學意義(χ~2=10.412,P<0.05).結論 NIP的髮病與HPV感染密切相關,HPV高危亞型可能促使NIP嚮不典型增生髮展.明確NIP中HPV亞型的分佈情況對腫瘤臨床行為的預測有一定指導意義.
목적 탐토인유두상류병독(human papillomavims,HPV)재비내번성유두상류(nasal inverted papilloma,NIP)중감염아형적분포급기재병변발생、발전중적작용.방법 취101례NIP석사포매조직표본(기중단순NIP조77례,반불전형증생NIP조22례,NIP암변조2례),채용핵산분자쾌속도류잡교기인심편분형기술(HybriMax)대21충HPV아형(병구분고위아형화저위아형)동보진행검측화병독분형,병이24례건강지원자비강점막조직위건강대조조.결과 NIP석사포매조직표본HPV총양성솔위64.4%(65/101),대조조HPV균위음성(0/24),량조비교차이유통계학의의(χ~2=32.178,P<0.05).단순NIP조、반불전형증생NIP조、NIP암변조HPV양성솔분별위59.7%(46/77)、81.8%(18/22)화50.0%(1/2),단순NIP조여반불전형증생NIP조비교,HPV양성솔차이무통계학의의(χ~2=3.649,P=0.056).단순NIP조여반불전형증생NIP조적HPV고위아형、저위아형적구성비차이유통계학의의(χ~2=10.412,P<0.05).결론 NIP적발병여HPV감염밀절상관,HPV고위아형가능촉사NIP향불전형증생발전.명학NIP중HPV아형적분포정황대종류림상행위적예측유일정지도의의.
Objective To study the distribution of human papillomavirus (HPV) subtypes in nasal inverted papilloma (NIP) ,and to evaluate tlle relationship between HPV and NIP.Methods Twenty-one HPV subtypes were detected in parafffin-embedded tissues of 101 cases of NIP by flow through hybridization and gene chip (HybriMax) ,24 cases of normal nagal mucosa were used as controls.Results HPV positive rates of NIP were 64.36% (65/101) .Benign NIP group,NIP with atypical hyperplasia group,NIP with Cancerous group of HPV positive rates were 59.7% (46/77) ,81.8% (18/22) and 50% (1/2) respectively.The control group was negative (0/24) .The comparison between NIP group and control group was statistically significant (χ~2=32.178,P<0.05) .Benign NIP group and NIP with atypical hyperplasia group were compared,but no statistically significance (χ~2=3.649,P=0.056) was found.The constituent ratio of benign NIP group and NIP with atypical hyperplfigia group in high,low-risk HPV subtypes infections was compared,a statistically significance (χ~2=10.412,P<0.05) was found.Conclusions The occurrence of NIP was related with HPV infection.H Jish-risk HPV subtype infections or multiple infections will prompt benign NIP to NIP with atypical hyperplasia.Understanding the distribution of HPV subtypes in the NIP is helpful to predict the clinical behavior.
