中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2009年
3期
136-139
,共4页
微核试验%彗星试验%DNA突变分析%炭黑
微覈試驗%彗星試驗%DNA突變分析%炭黑
미핵시험%혜성시험%DNA돌변분석%탄흑
Micronucleus tests%Comet assay%DNA mutational analysis%Carbon black
目的 评价2种粒径炭黑诱发的人B淋巴母细胞的遗传损伤效应.方法 人B淋巴母细胞经终浓度为0(溶剂对照)、128、256、384、512 ug/ml的14、280 nm炭黑颗粒染毒24、48 h后,用微核试验、hprt基因突变试验和彗星试验进行检测.微核试验指标为微核率(MNR)、微核细胞率(MCR)、核芽(Buds)、核质桥(NPBs)、核分裂指数(NDI)和凋亡细胞.彗星试验指标为尾部DNA百分比(%tail ONA)和olive尾矩(OTM).hprt基因突变试验指标为基因突变率(Mf-hprt).结果 14 nm炭黑染毒48 h组,浓度为384、512 ug/ml时,%tail DNA、OTM分别为8.23%±0.19%、11.23%±0.42%和3.72±0.08、4.90±0.18,明显高于对照组(5.10%±0.08%和2.22±0.03),差异有统计学意义(P<0.01);凋亡细胞数分别为4.67±0.33、5.33±0.33,明显高于对照组(0.00±0.00),差异有统计学意义(P<0.05).hprt 基因突变试验结果 呈阴性.结论 14 nm超细炭黑细颗粒染毒48 h可诱发人B淋巴母细胞DNA损伤,但280 nm的炭黑颗粒未检测出类似效应.
目的 評價2種粒徑炭黑誘髮的人B淋巴母細胞的遺傳損傷效應.方法 人B淋巴母細胞經終濃度為0(溶劑對照)、128、256、384、512 ug/ml的14、280 nm炭黑顆粒染毒24、48 h後,用微覈試驗、hprt基因突變試驗和彗星試驗進行檢測.微覈試驗指標為微覈率(MNR)、微覈細胞率(MCR)、覈芽(Buds)、覈質橋(NPBs)、覈分裂指數(NDI)和凋亡細胞.彗星試驗指標為尾部DNA百分比(%tail ONA)和olive尾矩(OTM).hprt基因突變試驗指標為基因突變率(Mf-hprt).結果 14 nm炭黑染毒48 h組,濃度為384、512 ug/ml時,%tail DNA、OTM分彆為8.23%±0.19%、11.23%±0.42%和3.72±0.08、4.90±0.18,明顯高于對照組(5.10%±0.08%和2.22±0.03),差異有統計學意義(P<0.01);凋亡細胞數分彆為4.67±0.33、5.33±0.33,明顯高于對照組(0.00±0.00),差異有統計學意義(P<0.05).hprt 基因突變試驗結果 呈陰性.結論 14 nm超細炭黑細顆粒染毒48 h可誘髮人B淋巴母細胞DNA損傷,但280 nm的炭黑顆粒未檢測齣類似效應.
목적 평개2충립경탄흑유발적인B림파모세포적유전손상효응.방법 인B림파모세포경종농도위0(용제대조)、128、256、384、512 ug/ml적14、280 nm탄흑과립염독24、48 h후,용미핵시험、hprt기인돌변시험화혜성시험진행검측.미핵시험지표위미핵솔(MNR)、미핵세포솔(MCR)、핵아(Buds)、핵질교(NPBs)、핵분렬지수(NDI)화조망세포.혜성시험지표위미부DNA백분비(%tail ONA)화olive미구(OTM).hprt기인돌변시험지표위기인돌변솔(Mf-hprt).결과 14 nm탄흑염독48 h조,농도위384、512 ug/ml시,%tail DNA、OTM분별위8.23%±0.19%、11.23%±0.42%화3.72±0.08、4.90±0.18,명현고우대조조(5.10%±0.08%화2.22±0.03),차이유통계학의의(P<0.01);조망세포수분별위4.67±0.33、5.33±0.33,명현고우대조조(0.00±0.00),차이유통계학의의(P<0.05).hprt 기인돌변시험결과 정음성.결론 14 nm초세탄흑세과립염독48 h가유발인B림파모세포DNA손상,단280 nm적탄흑과립미검측출유사효응.
Objective To assess the genetic damage of human B lymphocyte cell line induced by 14 nm and 280 nm carbon black (CB) particles with micronucleus assay (CBMN), comet assay and hprt gene mutation test in vitro. Methods The genetic damage of human B lymphocyte cells exposed to 14 nm and 280 nm CB particles at the doses of 0, 128, 256, 384 and 512 ug/ml for 24 h and 48 h was detected using above three genotoxic assays. Micronucleus (MN) assay, comet assay, hprt gene mutation test were used to detect the genetic damage of human B lymphocyte cells induced by CB. Micronucleus rate(MNR), micronu-cleated cell rate(MCR), nuclear buds (Buds), nucleoplasmic bridges(NPBs), nuclear division index (NDI) and numbers of apoptotic cells served as indexes of CBMN assay; the percentage of DNA in the tail(% tail DNA) and the alive tail moment (OTM) were used as DNA damage indicators of comet assay; the hprt gene muta-tion frequency (Mf-hprt) served as the index of hprt gene mutation test. Results The % tail DNA, OTM in 14 nm CB group at the doses of 384 and 512 ug/ml for 48 h were 8.23%±0.19%, 11.23%±0.42% and 3.72± 0.08,4.90±0.18, respectively, which were significantly higher than those in control (5.10%±0.08% and 2.22± 0.03) (P<0.01). The apoptotic cell rates in 14 nm CB group at the doses of 384 and 512 ug/ml for 48 h were 4.67±0.33 and 5.33±0.33, respectively,which were significantly higher than in control (0.00±0.00)(P<0.05). The results of Mf-hprt were negative. Conclusion The genetic damage of human B lymphocyte cells ex-posed to 14 nm CB particles for 48 h could be detected. But the similar effects didn't appear in 280 nm CB group.
