中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2010年
4期
300-303
,共4页
郭光华%王少根%付忠华%杨明%吴星恒%朱峰
郭光華%王少根%付忠華%楊明%吳星恆%硃峰
곽광화%왕소근%부충화%양명%오성항%주봉
烧伤,吸人性%高频通气%心肌%肌球蛋白轻链%肌球蛋白轻链激酶%半胱氨酸天冬氨酸蛋白酶1%常频机械通气
燒傷,吸人性%高頻通氣%心肌%肌毬蛋白輕鏈%肌毬蛋白輕鏈激酶%半胱氨痠天鼕氨痠蛋白酶1%常頻機械通氣
소상,흡인성%고빈통기%심기%기구단백경련%기구단백경련격매%반광안산천동안산단백매1%상빈궤계통기
Burns,inhalation%High-frequency ventilation%Myocardium%Myosin light chains%Myosin-light-chain kinase%Caspase 1%Conventional mandatory ventilation
目的 比较高频振荡通气(HFOV)和常频机械通气(CMV)对吸入性损伤兔心肌功能的影响. 方法 将16只新西兰大白兔制成蒸气吸入性损伤模型后,按随机抽签法分为CMV组和HFOV组,每组8只,分别行定容通气和HFOV治疗.4 h后处死2组家兔,处死前经心脏采血,检测血浆中乳酸脱氢酶1(LDH1)和心肌型肌酸磷酸激酶同工酶(CPK-MB)活性.取家兔左心室部分组织并匀浆,用ELISA法检测TNF-α、IL-8含量,分光光度法测定半胱氨酸天冬氨酸蛋白酶1(caspase-1)活性;核素液体闪烁技术测定心肌组织肌球蛋白轻链(MLC)-ATP酶和肌球蛋白轻链激酶(MLCK)活性.部分左心室心肌组织送病理检查.对实验数据行方差分析. 结果 (1)CMV组家兔血浆LDH1、CPK-MB活性分别为(643±108)、(342±48)U,明显高于HFOV组此2项指标[(233±92)、(186±36)U,F值分别为10.326、9.846,P值均小于0.01].(2)CMV组家兔心肌组织匀浆中TNF-α、IL-8含量和caspase-1活性分别为(181±35)pg/g、(89±19)pg/g、(0.56±0.27)g/g蛋白,均明显高于HFOV组[(94±21)pg/g、(43±11)pg/g、(0.24±0.12)g/g蛋白,F值分别为8.239、7.826、5.716,P值均小于0.01].(3)CMV组心肌组织MLC-ATP酶、MLCK活性分别为(0.24±0.12)μmo1·mg-1·min-1、(3.3±1.1)mmol·mg-1·min-1,均明显低于HFOV组[(0.48±0.16)μmol·mg-1·min-1、(7.7±1.7)mmol·mg-1·min-1,F值分别为4.125、4.766,P值均小于0.01].(4)光学显微镜下可见,2组家兔心肌组织无明显坏死、变性及炎性细胞浸润现象,但心肌纤维略显肿胀,HFOV组改变不及CMV组明显. 结论 与CMV模式比较,HFOV模式对吸人性损伤兔心肌肌球蛋白磷酸化系统影响较小.
目的 比較高頻振盪通氣(HFOV)和常頻機械通氣(CMV)對吸入性損傷兔心肌功能的影響. 方法 將16隻新西蘭大白兔製成蒸氣吸入性損傷模型後,按隨機抽籤法分為CMV組和HFOV組,每組8隻,分彆行定容通氣和HFOV治療.4 h後處死2組傢兔,處死前經心髒採血,檢測血漿中乳痠脫氫酶1(LDH1)和心肌型肌痠燐痠激酶同工酶(CPK-MB)活性.取傢兔左心室部分組織併勻漿,用ELISA法檢測TNF-α、IL-8含量,分光光度法測定半胱氨痠天鼕氨痠蛋白酶1(caspase-1)活性;覈素液體閃爍技術測定心肌組織肌毬蛋白輕鏈(MLC)-ATP酶和肌毬蛋白輕鏈激酶(MLCK)活性.部分左心室心肌組織送病理檢查.對實驗數據行方差分析. 結果 (1)CMV組傢兔血漿LDH1、CPK-MB活性分彆為(643±108)、(342±48)U,明顯高于HFOV組此2項指標[(233±92)、(186±36)U,F值分彆為10.326、9.846,P值均小于0.01].(2)CMV組傢兔心肌組織勻漿中TNF-α、IL-8含量和caspase-1活性分彆為(181±35)pg/g、(89±19)pg/g、(0.56±0.27)g/g蛋白,均明顯高于HFOV組[(94±21)pg/g、(43±11)pg/g、(0.24±0.12)g/g蛋白,F值分彆為8.239、7.826、5.716,P值均小于0.01].(3)CMV組心肌組織MLC-ATP酶、MLCK活性分彆為(0.24±0.12)μmo1·mg-1·min-1、(3.3±1.1)mmol·mg-1·min-1,均明顯低于HFOV組[(0.48±0.16)μmol·mg-1·min-1、(7.7±1.7)mmol·mg-1·min-1,F值分彆為4.125、4.766,P值均小于0.01].(4)光學顯微鏡下可見,2組傢兔心肌組織無明顯壞死、變性及炎性細胞浸潤現象,但心肌纖維略顯腫脹,HFOV組改變不及CMV組明顯. 結論 與CMV模式比較,HFOV模式對吸人性損傷兔心肌肌毬蛋白燐痠化繫統影響較小.
목적 비교고빈진탕통기(HFOV)화상빈궤계통기(CMV)대흡입성손상토심기공능적영향. 방법 장16지신서란대백토제성증기흡입성손상모형후,안수궤추첨법분위CMV조화HFOV조,매조8지,분별행정용통기화HFOV치료.4 h후처사2조가토,처사전경심장채혈,검측혈장중유산탈경매1(LDH1)화심기형기산린산격매동공매(CPK-MB)활성.취가토좌심실부분조직병균장,용ELISA법검측TNF-α、IL-8함량,분광광도법측정반광안산천동안산단백매1(caspase-1)활성;핵소액체섬삭기술측정심기조직기구단백경련(MLC)-ATP매화기구단백경련격매(MLCK)활성.부분좌심실심기조직송병리검사.대실험수거행방차분석. 결과 (1)CMV조가토혈장LDH1、CPK-MB활성분별위(643±108)、(342±48)U,명현고우HFOV조차2항지표[(233±92)、(186±36)U,F치분별위10.326、9.846,P치균소우0.01].(2)CMV조가토심기조직균장중TNF-α、IL-8함량화caspase-1활성분별위(181±35)pg/g、(89±19)pg/g、(0.56±0.27)g/g단백,균명현고우HFOV조[(94±21)pg/g、(43±11)pg/g、(0.24±0.12)g/g단백,F치분별위8.239、7.826、5.716,P치균소우0.01].(3)CMV조심기조직MLC-ATP매、MLCK활성분별위(0.24±0.12)μmo1·mg-1·min-1、(3.3±1.1)mmol·mg-1·min-1,균명현저우HFOV조[(0.48±0.16)μmol·mg-1·min-1、(7.7±1.7)mmol·mg-1·min-1,F치분별위4.125、4.766,P치균소우0.01].(4)광학현미경하가견,2조가토심기조직무명현배사、변성급염성세포침윤현상,단심기섬유략현종창,HFOV조개변불급CMV조명현. 결론 여CMV모식비교,HFOV모식대흡인성손상토심기기구단백린산화계통영향교소.
Objective To compare the effect of high frequency oscillatory ventilation (HFOV) and conventional mandatory ventilation (CMV) on the myocardial function of rabbits with inhalation injury.Methods Steam inhalation injury model was reproduced in 16 New Zealand albino rabbits. They were randomly divided into CMV group(n =8) and HFOV group(n =8) by drawing lots, and they received ventilation in metered volume and HFOV treatment respectively. Heart blood was drawn from rabbits before they were sacrificed 4 hours after treatment to determine the plasma activity of lactate dehydrogenase 1(LDH1)and creatine phosphorylated kinase (CPK-MB). Myocardial tissue from left ventricle was harvested and homogenized to determine the concentration of TNF-α and IL-8, the activity of caspase-1, and the activity of myosin-light-chain kinase (MLCK) and the ATPase of myosin light chain (MLC-ATPase) by enzyme-linked immunosorbent assay, spectrophotometry, and the nuclide liquid scintillation technique respectively. Part of the myocardial tissue sample was examined pathologically. Data were processed with analysis of variance.Results (1) The activities of LDH1 and CPK-MB in plasma were obviously higher in CMV group than in HFOV group [(643 ± 108), (342 ±48) U vs. (233 ±92),(186 ± 36) U, with F value respectively 10. 326 and 9. 846, P values all below 0.01]. (2) The contents of TNF-αt, IL-8 and the activity of caspase-1in myocardial tissue homogenate were obviously higher in CMV group than in HFOV group [(181 ± 35) ,(89±19) pg/g, and (0.56±0.27) g/g protein vs. (94±21), (43 ±11) pg/g, and (0.24±0.12) g/gprotein, with F value respectively 8. 239, 7. 826, 5. 716, P values all below 0. 01]. (3) The activities of MLC-ATPase and MLCK were lower in CMV group than in HFOV group [(0.24 ±0. 12) μmol ·mg-1min-1, (3.3± 1.1) mmol· mg-1 · min-1 vs. (0.48 ±0. 16) μmol ·mg-1 · min-1, (7.7 ± 1.7)mmol· mg-1 · min - 1, with F value respectively 4. 125, 4. 766, P values all below 0. 01]. (4) No obvious necrosis, degeneration or inflammatory cell infiltration was observed in myocardial tissue of rabbits in 2groups under light microscope; but the myocardial fiber was slightly swollen, and it was less marked in the HFOV group. Conclusions The influence of HFOV on myocardial myosin phosphorylation system of rabbits with inhalation injury is less than that of CMV.
