CAJ | 학술논문

目的了解河北某非法采血村村民感染丙型肝炎病毒(HCV)基因亚型分布情况.方法对该村全体村民采集静脉血标本计520份,无菌分离血清;以RT-PCR法扩增其中可利用的483份血清HCV之C/E1基因片段,双脱氧链终止法测序;用Mega4.0软件进行HCV系统进化分析,构建系统进化树,判定基因亚型.结果 483份标本中,HCV RNA阳性者70份,阳性率14.5%;基因分型1b亚型36例,占51.4%;2a亚型34例,占48.6%.结论该村村民血清HCV检出率约为14.5%,远高于一般人群;HCV亚型构成为1b、2a各半.
목적 료해하북모비법채혈촌촌민감염병형간염병독(HCV)기인아형분포정황.방법 대해촌전체촌민채집정맥혈표본계520빈,무균분리혈청;이RT-PCR법확증기중가이용적483빈혈청HCV지C/E1기인편단,쌍탈양련종지법측서;용Mega4.0연건진행HCV계통진화분석,구건계통진화수,판정기인아형.결과 483빈표본중,HCV RNA양성자70빈,양성솔14.5%;기인분형1b아형36례,점51.4%;2a아형34례,점48.6%.결론 해촌촌민혈청HCV검출솔약위14.5%,원고우일반인군;HCV아형구성위1b、2a각반.
Objective To investigate genotype of HCV infection among residents in a rural village, Hebei Province. Methods Blood samples of the 520 residents were collected, and C/E1 of HCV genome of the 483 samples were obtained by RT-PCR amplifying, and the gene sequences were analyzed and the polygenetic tree were drawn by the software Mega 4.0. Results In 483 subjects, HCV-RNA positive objectives are 70, positive prevalence is 14.5%, genotyping for all the samples successfully, including genotypes 1b and 2a, which are 36 (51.4%),34(48.6%) respectively. Conclusion HCV RNA positive rate is 14.5%, which is higher than general people. Subtypes 1b and 2a seemed to be the dominant genotypes of HCV in Zhao county area of Hebei Province.