CAJ | 학술논문

目的探讨胃癌组织、癌旁组织及淋巴结转移灶中Ets-1表达的临床意义,分析Ets-1表达与血管生成、临床病理特征及预后的关系.方法应用免疫组化SP法,采用组织芯片技术,检测189例胃癌组织、54例癌旁组织、41例淋巴结转移灶及32例正常胃黏膜中Ets-1蛋白的表达.对胃癌患者通过上门或电话进行问卷随访.结果胃癌组织、癌旁组织和正常胃黏膜Ets-1的阳性表达率分别为71.4%、29.6%和18.8%,3组间差异有统计学意义(P＜0.01).135例Ets-1表达阳性的胃癌组织微血管密度(MVD)为30.42±15.21,54例Ets-1表达阴性的胃癌组织MVD为25.73±11.50.两组差异有统计学意义(P=0.042).Ets-1蛋白表达与浸润深度、淋巴结转移有关(P＜0.01),与性别、年龄、肿瘤大小、分化程度、Lanren分型无关(P＞0.05).41例淋巴结转移灶和相对应的41例胃癌组织Ets-1表达阳性率分别为84.4%和58.5%,差异有统计学意义(P=0.007).单因素分析显示,Ets-1表达对胃癌患者生存期的影响有统计学意义(P＜0.05),Cox多元回归分析显示,Ets-1表达不是胃癌患者预后的独立影响因素(P＞0.05).结论 Ets-1在促进胃癌血管生成中发挥着重要作用,在胃癌的发生、发展中扮演了重要角色,Ets-1表达对胃癌患者的生存期有一定影响,胃癌淋巴结转移灶和原发灶肿瘤组织中Ets-1的表达不同,具有异质性.
목적 탐토위암조직、암방조직급림파결전이조중Ets-1표체적림상의의,분석Ets-1표체여혈관생성、림상병리특정급예후적관계.방법 응용면역조화SP법,채용조직심편기술,검측189례위암조직、54례암방조직、41례림파결전이조급32례정상위점막중Ets-1단백적표체.대위암환자통과상문혹전화진행문권수방.결과 위암조직、암방조직화정상위점막Ets-1적양성표체솔분별위71.4%、29.6%화18.8%,3조간차이유통계학의의(P＜0.01).135례Ets-1표체양성적위암조직미혈관밀도(MVD)위30.42±15.21,54례Ets-1표체음성적위암조직MVD위25.73±11.50.량조차이유통계학의의(P=0.042).Ets-1단백표체여침윤심도、림파결전이유관(P＜0.01),여성별、년령、종류대소、분화정도、Lanren분형무관(P＞0.05).41례림파결전이조화상대응적41례위암조직Ets-1표체양성솔분별위84.4%화58.5%,차이유통계학의의(P=0.007).단인소분석현시,Ets-1표체대위암환자생존기적영향유통계학의의(P＜0.05),Cox다원회귀분석현시,Ets-1표체불시위암환자예후적독립영향인소(P＞0.05).결론 Ets-1재촉진위암혈관생성중발휘착중요작용,재위암적발생、발전중분연료중요각색,Ets-1표체대위암환자적생존기유일정영향,위암림파결전이조화원발조종류조직중Ets-1적표체불동,구유이질성.
Objective To investigate the expression of Ets-I in gastric carcinoma,pars-cancerous tissue and metastatic lymph nodes,and to determine the relationship between Ets-1 expression and clinicopathological features,angiogenesis and survival of patients with gastric carcinoma.Methods Gastric carcinoma tissue microarray was used to determine Ets-I protein expression by SP immunohistochemical staining in 189 advanced gastric cancer,54 papacancerous tissues,41 metastatic lymph nodes and 32 control tissues.Results The positive rates for Ets-1 expression of the carcinoma,paracancerous and control tissues were 71.4 %,29.6% and 18.8%,respectively,with a significant difference among the three groups(P ＜0.01).In the cancer tissues,the positive rate of Ets-1 protein expression was significantly associated with depth of invasion and lymph node metastasis(P ＜0.01),but not associated with degree of differentiation,Lauren's histological type,sex,age,and size of tumor(P ＞0.05).The positive rates for Ets-1 expression of the 41 gastric cancer and 41 metastatic lymph nodes were significantly different(P ＜0.05).In metastatic lymph nodes,the positive rate for Ets-1 expression was higher.The MVD in Ets-1 positive tumors was higher than that in the Ets-1 negative tumors,with a significant difference(P ＜ 0.05).Kaplan-Meier survival analysis showed that the survival time of Ets-1-negative patients was longer than that of Ets-1-positive patients (P ＜0.05).Cox regression analysis showed that Ets-1 expression was not an independent prognostic factor of gastric carcinoma.Conclusion A higher expression of Ets-1 is involved in carcinogenesis,development,invasion,and metastasis of gastric cancer.Ets-1 plays an important role in angiogenesis in gastric cancer.Ets-1 is a useful marker for predicting the outcome for patients with gastric carcinoma,though it is not an independent prognostic indicator.