中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
5期
583-586
,共4页
张心菊%顾小叶%刘双春%马玮哲%关明
張心菊%顧小葉%劉雙春%馬瑋哲%關明
장심국%고소협%류쌍춘%마위철%관명
骨髓增殖性疾病%白血病%Janus激酶2%突变%聚合酶链反应
骨髓增殖性疾病%白血病%Janus激酶2%突變%聚閤酶鏈反應
골수증식성질병%백혈병%Janus격매2%돌변%취합매련반응
Myeloproliferative disorders%Leukemia%Janus kinase 2%Mutation%Polymerase chain reaction
目的 建立荧光定量PCR检测JAK2基因V617F突变的方法,评估JAK2 V617F突变在诊断骨髓增殖性疾病和白血病中的临床意义.方法 选取71例慢性粒细胞性白血病(CML)、22例原发性血小板增多症(ET)、11例原发性骨髓纤维化(PMF)、9例真性红细胞增多症(PV)、7例嗜酸粒细胞增多症患者,分别采用荧光定量PCR、突变特异性扩增系统(ARMS)对JAK2 V617F突变进行检测,并采用基因测序对结果进行验证.将具有JAK2 V617F纯合子突变的人类红白血病细胞株(HEL)DNA作为阳性对照,比较荧光定量PCR和ARMS对JAK2基因V617F突变的检测敏感度.结果 采用荧光定量PCR检测,野生型的熔解温度(Tm)峰出现于(75.0±0.2)℃处,突变型的Tm峰出现于(76.6±0.2)℃处.JAK2基因V617F突变在PV、ET、PMF等骨髓增殖性疾病中的检出率分别为8例(88.9%)、12例(54.5%)、7例(63.6%),但在71例CML中只检出1例(1.4%).荧光定量PCR与ARMS的结果符合率为100%,结果经过测序证实.使用荧光定量PER法可在每106个正常自细胞中检出低至102个HEL细胞,而使用ARMS方法时要在每106个正常白细胞中HEL细胞达到104个时,方可检测出,前者比后者方法灵敏100倍.结论 成功地建立了荧光定量PCR检测JAK2基因V617F突变的方法,比ARMS更灵敏、简便,适合临床使用.JAK2基因V617F突变在骨髓增殖性疾病中有较高的检出率,可作为骨髓增殖性疾病特异性诊断指标.
目的 建立熒光定量PCR檢測JAK2基因V617F突變的方法,評估JAK2 V617F突變在診斷骨髓增殖性疾病和白血病中的臨床意義.方法 選取71例慢性粒細胞性白血病(CML)、22例原髮性血小闆增多癥(ET)、11例原髮性骨髓纖維化(PMF)、9例真性紅細胞增多癥(PV)、7例嗜痠粒細胞增多癥患者,分彆採用熒光定量PCR、突變特異性擴增繫統(ARMS)對JAK2 V617F突變進行檢測,併採用基因測序對結果進行驗證.將具有JAK2 V617F純閤子突變的人類紅白血病細胞株(HEL)DNA作為暘性對照,比較熒光定量PCR和ARMS對JAK2基因V617F突變的檢測敏感度.結果 採用熒光定量PCR檢測,野生型的鎔解溫度(Tm)峰齣現于(75.0±0.2)℃處,突變型的Tm峰齣現于(76.6±0.2)℃處.JAK2基因V617F突變在PV、ET、PMF等骨髓增殖性疾病中的檢齣率分彆為8例(88.9%)、12例(54.5%)、7例(63.6%),但在71例CML中隻檢齣1例(1.4%).熒光定量PCR與ARMS的結果符閤率為100%,結果經過測序證實.使用熒光定量PER法可在每106箇正常自細胞中檢齣低至102箇HEL細胞,而使用ARMS方法時要在每106箇正常白細胞中HEL細胞達到104箇時,方可檢測齣,前者比後者方法靈敏100倍.結論 成功地建立瞭熒光定量PCR檢測JAK2基因V617F突變的方法,比ARMS更靈敏、簡便,適閤臨床使用.JAK2基因V617F突變在骨髓增殖性疾病中有較高的檢齣率,可作為骨髓增殖性疾病特異性診斷指標.
목적 건립형광정량PCR검측JAK2기인V617F돌변적방법,평고JAK2 V617F돌변재진단골수증식성질병화백혈병중적림상의의.방법 선취71례만성립세포성백혈병(CML)、22례원발성혈소판증다증(ET)、11례원발성골수섬유화(PMF)、9례진성홍세포증다증(PV)、7례기산립세포증다증환자,분별채용형광정량PCR、돌변특이성확증계통(ARMS)대JAK2 V617F돌변진행검측,병채용기인측서대결과진행험증.장구유JAK2 V617F순합자돌변적인류홍백혈병세포주(HEL)DNA작위양성대조,비교형광정량PCR화ARMS대JAK2기인V617F돌변적검측민감도.결과 채용형광정량PCR검측,야생형적용해온도(Tm)봉출현우(75.0±0.2)℃처,돌변형적Tm봉출현우(76.6±0.2)℃처.JAK2기인V617F돌변재PV、ET、PMF등골수증식성질병중적검출솔분별위8례(88.9%)、12례(54.5%)、7례(63.6%),단재71례CML중지검출1례(1.4%).형광정량PCR여ARMS적결과부합솔위100%,결과경과측서증실.사용형광정량PER법가재매106개정상자세포중검출저지102개HEL세포,이사용ARMS방법시요재매106개정상백세포중HEL세포체도104개시,방가검측출,전자비후자방법령민100배.결론 성공지건립료형광정량PCR검측JAK2기인V617F돌변적방법,비ARMS경령민、간편,괄합림상사용.JAK2기인V617F돌변재골수증식성질병중유교고적검출솔,가작위골수증식성질병특이성진단지표.
Objective To establish real-time PCR method for the detection of JAK2 V617F mutation and evaluate its clinical significance in patients with myeloprollferative disorders and leukemia.Methods 71 chronic myelocytic leukemia(CML) patients, 22 essential thrombocythemia (ET) patients, 11 primary myelofibrosis (PMF) patients, 9 polycythemia vera (PV) patients and 7 cosinophilia patients were enrolled in this study. JAK2 V617F mutation was determined by real-time PCR and amplification refractory mutation system (ARMS), followed by sequencing. Human erythroleukemia cell (HEL cell)DNA was used as homozygous control of JAK2 V617F mutation. The detection limit for either real-time PCR or ARMS was evaluated. Results Real-time PCR assay showed that there was a melting temperature(Tin) peak at (75.0±0.2)℃ for wild type samples and a Tm peak at (76.6±0.2)℃ for mutation type samples. JAK2 V617F mutation was detected in 8(88.9%) patients with PV, 12(54.5%) patients with ET and 7(63.6%) patients with PMF respectively. But there was only one positive case in 71 CML patient (1.4%). The results showed complete concordance with ARMS results and confirmed by sequencing. The mutation could be detected in 102 HEL cells per 106 white blood cells by real-time PCR, whereas the mutation can be assessed in 104 HEL cells per 106 white blood cells by ARMS. Thus, the sensitivity of real-time PCR was 100-fold higher than ARMS. Conclusions The real-time PCR method is successfully established for detection of JAK2 V617F mutation. This method is more sensitive, convenient than ARMS, and suitable for clinical application. There is high frequency of JAK2 V617F mutation in myeloproliferative disorders and it could be used as the diagnostic marker for myeloproliferative disorders.