CAJ | 학술논문

本文报道了口蹄疫病毒（Foot-and-Mouth Disease Virus, FMDV）在体外诱导PK-15细胞凋亡的研究结果。采用Hoechst33258荧光探针、DNA凝胶电泳、脱氧核糖核酸转移酶介导的缺口末端标记（TUNEL）技术均检测到了典型的细胞凋亡。结果显示：使用感染性滴度为4.8 lgTCID50/mL的口蹄疫病毒感染PK-15细胞，在培养32?h 后荧光探针检测呈现典型的凋亡细胞核固缩和梅花状碎裂核，并伴随有凋亡小体出现，凋亡率约为20%；DNA凝胶电泳显示ladder梯带；末端标记检测到强绿色荧光标记物结合于凋亡细胞核上。研究结果提示：口蹄疫病毒可以在体外诱导宿主细胞凋亡，细胞凋亡是其致细胞病变死亡的重要途径之一。
본문보도료구제역병독（Foot-and-Mouth Disease Virus, FMDV）재체외유도PK-15세포조망적연구결과。채용Hoechst33258형광탐침、DNA응효전영、탈양핵당핵산전이매개도적결구말단표기（TUNEL）기술균검측도료전형적세포조망。결과현시：사용감염성적도위4.8 lgTCID50/mL적구제역병독감염PK-15세포，재배양32?h 후형광탐침검측정현전형적조망세포핵고축화매화상쇄렬핵，병반수유조망소체출현，조망솔약위20%；DNA응효전영현시ladder제대；말단표기검측도강록색형광표기물결합우조망세포핵상。연구결과제시：구제역병독가이재체외유도숙주세포조망，세포조망시기치세포병변사망적중요도경지일。
Apoptosis of PK-15 cells induced by Foot-and-Mouth Disease Virus (FMDV) in vitro was reported in this paper. Typical cell apoptosis was detected by use of Hoechst 33258 fluorescence probe, agarose gel electrophoresis and in situ end-labeling (TUNEL). After PK-15 cells were infected by titration of 4.8 lg TCID50/mL FMDV for 32 h, apoptosis characteristics of nuclear condensation, fragmentation, accompanied by apoptotic bodies formation (Hoechst 33258 staining), 180-200 integer-fold sized pieces DNA Ladders (agarose gel electrophoresis) and strong green fluorescence dots (TUNEL) were all exhibited, and cell apoptosis was approximately 20%. In addition, the quantitative analysis of apoptosis in PK-15 cells induced by FMDV showed that apoptosis was correlated with infection of virus, and it was also time-dependent. Results indicate that FMDV can induce apoptosis of host cells and apoptosis plays an important role in the cytopathogencity effect of FMDV.