CAJ | 학술논문

背景:鸡冠花黄酮类化合物是一种植物性雌激素,具有多种生理功能,且无毒副作用,对骨质疏松的治疗和预防都具有良好干预效果.目的:探讨鸡冠花黄酮化合物对糖尿病大鼠骨形成蛋白、尿无机盐以及溶菌酶含量的影响.设计:随机区组分组设计,设立对照实验.单位:新乡医学院药物研究室.材料:实验于2003-09/12在新乡医学院完成.选用健康雌性SD大鼠24只.按区组随机法分组法将大鼠分为3组:正常对照组,糖尿病模型组,糖尿病+鸡冠花黄酮组,每组8只.方法:①糖尿病+鸡冠花黄酮组和糖尿病模型组大鼠腹腔注射链脲佐菌素60 mg/kg,48～72 h后尾静脉采血,测定全血血糖,血糖≥16.7 mmol/L确定为糖尿病大鼠,血糖未达16.7 mmol/L者立即腹腔补注链脲佐菌素60 mg/kg.造模后糖尿病+鸡冠花黄酮组给予鸡冠花黄酮100 mg/(kg·d)灌胃,正常对照组仅注射等量生理盐水.②各组干预10周后,采用原子吸光光度法测定大鼠尿钙含量,火焰分光光度法测尿钠、钾含量.③采用过氧化物酶标记的链霉卵白素法按照说明书进行骨形成蛋白2免疫组织化学测定,用HPIAS-2000图像分析仪对各组骨形成蛋白2免疫组织化学表达进行定量分析.④采用微量溶菌酶比色法测定反映肾小管的重吸收功能的溶菌酶含量.⑤组间差异比较用t检验.主要观察指标:各组干预10周后,大鼠尿钙、钠、钾及溶菌酶含量及骨形成蛋白2免疫组织化学表达比较.结果:SD大鼠24只均进入结果分析.①糖尿病模型组大鼠的尿钙、钠含量明显高于正常对照组,尿钾显著低于正常对照组(P＜0.05～0.01).糖尿病+鸡冠花黄酮组大鼠尿钙含量明显低于糖尿病模型组(P＞0.05).②糖尿病+鸡冠花黄酮组和正常对照组大鼠骨组织骨形成蛋白2表达明显高于糖尿病模型组,尿溶菌酶含量明显低于糖尿病模型组(P＜0.05～0.01).结论:糖尿病大鼠骨组织骨形成蛋白2表达明显下降,补充鸡冠花黄酮类化合物后动物骨骨形成蛋白2表达升高,糖尿病大鼠的尿钙和尿钠的排出显著降低,肾小管的重吸收功能提高. 揹景:鷄冠花黃酮類化閤物是一種植物性雌激素,具有多種生理功能,且無毒副作用,對骨質疏鬆的治療和預防都具有良好榦預效果.目的:探討鷄冠花黃酮化閤物對糖尿病大鼠骨形成蛋白、尿無機鹽以及溶菌酶含量的影響.設計:隨機區組分組設計,設立對照實驗.單位:新鄉醫學院藥物研究室.材料:實驗于2003-09/12在新鄉醫學院完成.選用健康雌性SD大鼠24隻.按區組隨機法分組法將大鼠分為3組:正常對照組,糖尿病模型組,糖尿病+鷄冠花黃酮組,每組8隻.方法:①糖尿病+鷄冠花黃酮組和糖尿病模型組大鼠腹腔註射鏈脲佐菌素60 mg/kg,48～72 h後尾靜脈採血,測定全血血糖,血糖≥16.7 mmol/L確定為糖尿病大鼠,血糖未達16.7 mmol/L者立即腹腔補註鏈脲佐菌素60 mg/kg.造模後糖尿病+鷄冠花黃酮組給予鷄冠花黃酮100 mg/(kg·d)灌胃,正常對照組僅註射等量生理鹽水.②各組榦預10週後,採用原子吸光光度法測定大鼠尿鈣含量,火燄分光光度法測尿鈉、鉀含量.③採用過氧化物酶標記的鏈黴卵白素法按照說明書進行骨形成蛋白2免疫組織化學測定,用HPIAS-2000圖像分析儀對各組骨形成蛋白2免疫組織化學錶達進行定量分析.④採用微量溶菌酶比色法測定反映腎小管的重吸收功能的溶菌酶含量.⑤組間差異比較用t檢驗.主要觀察指標:各組榦預10週後,大鼠尿鈣、鈉、鉀及溶菌酶含量及骨形成蛋白2免疫組織化學錶達比較.結果:SD大鼠24隻均進入結果分析.①糖尿病模型組大鼠的尿鈣、鈉含量明顯高于正常對照組,尿鉀顯著低于正常對照組(P＜0.05～0.01).糖尿病+鷄冠花黃酮組大鼠尿鈣含量明顯低于糖尿病模型組(P＞0.05).②糖尿病+鷄冠花黃酮組和正常對照組大鼠骨組織骨形成蛋白2錶達明顯高于糖尿病模型組,尿溶菌酶含量明顯低于糖尿病模型組(P＜0.05～0.01).結論:糖尿病大鼠骨組織骨形成蛋白2錶達明顯下降,補充鷄冠花黃酮類化閤物後動物骨骨形成蛋白2錶達升高,糖尿病大鼠的尿鈣和尿鈉的排齣顯著降低,腎小管的重吸收功能提高.
배경:계관화황동류화합물시일충식물성자격소,구유다충생리공능,차무독부작용,대골질소송적치료화예방도구유량호간예효과.목적:탐토계관화황동화합물대당뇨병대서골형성단백、뇨무궤염이급용균매함량적영향.설계:수궤구조분조설계,설립대조실험.단위:신향의학원약물연구실.재료:실험우2003-09/12재신향의학원완성.선용건강자성SD대서24지.안구조수궤법분조법장대서분위3조:정상대조조,당뇨병모형조,당뇨병+계관화황동조,매조8지.방법:①당뇨병+계관화황동조화당뇨병모형조대서복강주사련뇨좌균소60 mg/kg,48～72 h후미정맥채혈,측정전혈혈당,혈당≥16.7 mmol/L학정위당뇨병대서,혈당미체16.7 mmol/L자립즉복강보주련뇨좌균소60 mg/kg.조모후당뇨병+계관화황동조급여계관화황동100 mg/(kg·d)관위,정상대조조부주사등량생리염수.②각조간예10주후,채용원자흡광광도법측정대서뇨개함량,화염분광광도법측뇨납、갑함량.③채용과양화물매표기적련매란백소법안조설명서진행골형성단백2면역조직화학측정,용HPIAS-2000도상분석의대각조골형성단백2면역조직화학표체진행정량분석.④채용미량용균매비색법측정반영신소관적중흡수공능적용균매함량.⑤조간차이비교용t검험.주요관찰지표:각조간예10주후,대서뇨개、납、갑급용균매함량급골형성단백2면역조직화학표체비교.결과:SD대서24지균진입결과분석.①당뇨병모형조대서적뇨개、납함량명현고우정상대조조,뇨갑현저저우정상대조조(P＜0.05～0.01).당뇨병+계관화황동조대서뇨개함량명현저우당뇨병모형조(P＞0.05).②당뇨병+계관화황동조화정상대조조대서골조직골형성단백2표체명현고우당뇨병모형조,뇨용균매함량명현저우당뇨병모형조(P＜0.05～0.01).결론:당뇨병대서골조직골형성단백2표체명현하강,보충계관화황동류화합물후동물골골형성단백2표체승고,당뇨병대서적뇨개화뇨납적배출현저강저,신소관적중흡수공능제고.
BACKGROUND: Cristata L flavonoid is a kind of plant estrin, which possesses multiple physiological function, has no toxicity and adverse effect, and is effective in treating and preventing osteoporosis.OBJECTIVE: To study the effect of cristata L flavonoid on bone morphogenetic protein, urine inorganic salt and content of lysozyme of rats with diabetes mellitus (DM).DESIGN: Randomized grouping design and controlled study.SETTING: Pharmaceutical Laboratory of Xinxiang Medical College.MATERIALS: The experiment was completed in the Xinxiang Medical College from September to December 2003. Totally 24 health male SD rats were randomly divided into three groups: normal control group, diabetes mellitus (DM) group, DM + cristata L flavonoid group with 8 in each group.were injected intraperineally with 60 mg/kg streptozotocin, and 48-72 hours later, blood of rear caudal vein was collected to measure total blood glucose.Rats were determined as DM if the blood glucose was ≥ 16.7 mmol/L; oth erwise, 60 mg/kg streptozotocin was injected once more. After modeling,cristata L flavonoid and rats in normal control group were given the same was measured with atomic absorbency method and content of urine sodium histochemistry of bone morphogenic protein-2 (BMP2) in bone was detected with streptavidin tagged by peroxidase and immunohistochemic expression lysozyme reflected reabsorption function of renal tubule was measured with with t-test. MAIN OUTCOME MEASURES: Comparison between content of calcium, sodium, kalium in urine and lysozyme and immunohistochemic expression of BMP2 10-week intervention later.calcium and sodium in urine in DM model group were obviously higher than those in normal control group, but content of kalium in urine was obviously lower (P ＜ 0.05-0.01). Contents of calcium in urine in DM +cristata L flavonoid group were obvioualy lower than those in DM model group and normal control group were obviously higher than those in DM model group, and content of lysozyme in urine in those groups was obviously lower than that in DM model group (P ＜ 0.05-0.01).CONCLUSION: Expression of BMP2 of DM rats is decreased, but after supplement of cristata L flavonoid compound, the expression is increased.In addition, output of urine calcium and sodium in DM rats is decreased,and reabsorption function of renal tubule is increased.