CAJ | 학술논문

采用超声预处理强化紫外线消毒效果,并从分子生物学的角度探讨了其强化机理.结果表明,无论是单独紫外辐照还是超声预处理/紫外辐照,紫外辐照剂量与大肠杆菌灭活率之间均具有良好的线性关系;进行超声灭活时,超声功率、作用时间及菌悬液体积对灭活率均无显著意义(P>0.05),按影响大小排序为:菌悬液体积>超声功率>作用时间;当单独超声处理200 mL大肠杆菌悬液时,超声功率为200 W、作用时间为20 S条件下的灭活率最高(0.22-1g),该条件下继续进行紫外辐照,灭活率最高达到2.75-1g,1 h光复活后灭活率仍高达1.72-1g,超声/紫外协同作用的灭活率大于二者单独作用的灭活率之和,表明该条件下超声预处理起到了强化紫外线消毒的作用;超声处理只能改变细胞的结构,不能破坏其DNA;酶敏感位点数量与大肠杆菌的灭活率具有较强的相关性,相关系数约为0.88.
채용초성예처리강화자외선소독효과,병종분자생물학적각도탐토료기강화궤리.결과표명,무론시단독자외복조환시초성예처리/자외복조,자외복조제량여대장간균멸활솔지간균구유량호적선성관계;진행초성멸활시,초성공솔、작용시간급균현액체적대멸활솔균무현저의의(P>0.05),안영향대소배서위:균현액체적>초성공솔>작용시간;당단독초성처리200 mL대장간균현액시,초성공솔위200 W、작용시간위20 S조건하적멸활솔최고(0.22-1g),해조건하계속진행자외복조,멸활솔최고체도2.75-1g,1 h광복활후멸활솔잉고체1.72-1g,초성/자외협동작용적멸활솔대우이자단독작용적멸활솔지화,표명해조건하초성예처리기도료강화자외선소독적작용;초성처리지능개변세포적결구,불능파배기DNA;매민감위점수량여대장간균적멸활솔구유교강적상관성,상관계수약위0.88.
Ultrasound pretreatment was applied to strengthen the effect of ultraviolet (UV) disin-fection, and the mechanism was investigated based on molecular biology. The results show that whether for single UV irradiation or ultrasonic pretreatment/UV irradiation, there is a good linear relation between the UV irradiation dose and the inactivation rate of E. coli. During ultrasonic inactivation, the ultrasonic power, action time and volume of bacterial liquid have no statistical significance to inactivation rate (p> 0.05), and the arrangement in order of importance is the bacterial liquid volume > the ultrasonic power > the action time. When ultrasound is used to pretreat 200 mL E. coli liquid, the maximal inactivation rate is 0.22-1g under the conditions of 200 W ultrasonic power and 20 s action time. Under above condi-tions, when it is followed by UV irradiation, the maximal inactivation rate is 2.75-1g, even after 1 h pho-toreactivation, the inactivation rate remains at 1.72-1g. The inactivation rate in the synergistic system of ultrasound and UV is more than the total sum of the two separate inactivation rates, demonstrating that ul-trasonic pretreatment can strengthen the effect of UV disinfection. Ultrasound only can change the cell structure and can not destroy the DNA. There is positive relation between ESS and the inactivation of E. coli, and the correlation coefficient is about 0.88.