CAJ | 학술논문

[目的]通过筛杏猕猴桃EST数据库中的SSR重复序列,为开发出新型的猕猴桃EST-SSR标记和分子生物学研究奠定理论基础.[方法]从NCBI公共数据库中最新公布的猕猴桃表达序列标签(Expressed Sequence Tag,EST)中随机抽取56 400条序列,应用SSRHunter软件查找微卫星(Microsatellite,SSR)重复序列.搜索的标准是:二核苷酸、三核苷酸、四核苷酸、五核苷酸和六核苷酸最少重复次数分别为7次、5次、4次、4次、3次,包括复合型微卫星.微卫星的重复次数越多,相应的检验出等位基因数目就越多.[结果]从猕猴桃EST序列中获得了7 939条SSR,其中包括二核苷酸重复5 131条(64.63%),三核苷酸重复1 237条(15.58%),四核苷酸重复284条(3.58%),五核苷酸重复397条(5.00%),六核苷酸重复890条(11.21%).大约每2.48 kb长度的单一基因序列中即存在1个SSR,即平均7个单一基因中存在1个SSR.在二核苷酸重复序列中,AG/CT共分布4 654条(90.70%).在三核苷酸中,较为丰富的3种重复基元是ACC/GGT,AAG/CTT和CGC/GCG,共分布817条,它们占三核苷酸重复中各重复基元的66.04%.在其他基元中,还包括ACG/CCT,AGC/GCT,AAC/GTY,ATC/GAT,AGT/ACT,CGA/TCG,AAT/ATT 7种重复基元,共420条SSR序列,占33.96%.AGT与CGT重复基元未见分布.[结论]在猕猴桃EST序列中,二核苷酸重复序列是最丰富的重复单元,其次为三核苷酸重复和六核苷酸重复.在所获得的SSR重复单元中,AG/CT为优势重复.
[목적]통과사행미후도EST수거고중적SSR중복서렬,위개발출신형적미후도EST-SSR표기화분자생물학연구전정이론기출.[방법]종NCBI공공수거고중최신공포적미후도표체서렬표첨(Expressed Sequence Tag,EST)중수궤추취56 400조서렬,응용SSRHunter연건사조미위성(Microsatellite,SSR)중복서렬.수색적표준시:이핵감산、삼핵감산、사핵감산、오핵감산화륙핵감산최소중복차수분별위7차、5차、4차、4차、3차,포괄복합형미위성.미위성적중복차수월다,상응적검험출등위기인수목취월다.[결과]종미후도EST서렬중획득료7 939조SSR,기중포괄이핵감산중복5 131조(64.63%),삼핵감산중복1 237조(15.58%),사핵감산중복284조(3.58%),오핵감산중복397조(5.00%),륙핵감산중복890조(11.21%).대약매2.48 kb장도적단일기인서렬중즉존재1개SSR,즉평균7개단일기인중존재1개SSR.재이핵감산중복서렬중,AG/CT공분포4 654조(90.70%).재삼핵감산중,교위봉부적3충중복기원시ACC/GGT,AAG/CTT화CGC/GCG,공분포817조,타문점삼핵감산중복중각중복기원적66.04%.재기타기원중,환포괄ACG/CCT,AGC/GCT,AAC/GTY,ATC/GAT,AGT/ACT,CGA/TCG,AAT/ATT 7충중복기원,공420조SSR서렬,점33.96%.AGT여CGT중복기원미견분포.[결론]재미후도EST서렬중,이핵감산중복서렬시최봉부적중복단원,기차위삼핵감산중복화륙핵감산중복.재소획득적SSR중복단원중,AG/CT위우세중복.
[Objective]The aim was to make better use of the EST-SSR resources of kiwifruit for further molecular biological studies and new EST-SSR marker development by screening and mining the SSR repeats in the EST database of kiwifruit(Actinidia spp.)[Method]56 400 of EST sequences were randomly selected from EST(Expressed Sequence Tag)sequences of kiwifruit in the database of NCBI.EST sequences were analyzed and the SSR(Microsatellite)was screened by using the SSRHunter software.[Result]7 939 SSRs were identified from the randomly selected kiwifruit EST resources,among which there were 5 131(64.63%)dinucleotide,1 237(15.58%)trinucleotide,284(3.58%)tetra nucleotide,397(5.00%)pentanucleotide and 890(11.21 %)hexanucleotide SSRs.Among the dinucleotide sequences,AG/CT repeat motif was accounted for 4 654(90.70%).The frequency of SSRs was approximately 1/2.48 kb,which could exist to 1 SSR in 7 unigenes.[Conclusion]The dinucleotide repeats appeared to be the most abundant SSRs,followed by the trinucleotide and hexanucleotide repeats.Among them the repeat motif such as AG/CT was predominant in each type of SSRs.