CAJ | 학술논문

目的通过NMR定性研究大黄素的结构以及大黄素结构中的活泼性部位,为大黄素的化学结构修饰提供依据.方法从虎杖中高效提取了蒽醌类化合物大黄素,采用1D和2D-NMR的分析方法对大黄素结构进行鉴定,通过NMR变温技术及NOESY技术研究活泼性部位.结果以DMSO为溶剂、TMS作内标,采用~1HNMR,~(13)CNMR,DEPF,~1H-~1H COSY,NOESY,HSQC,HMBC等多种核磁共振方法对大黄素氢谱、碳谱进行完整归属,并通过NMR变温技术及NOESY的研究确定了其结构的活泼性部位.结论大黄素的3位羟基为其活泼性部位,该信息为大黄素的结构修饰以及衍生物的设计与抗肿瘤活性研究提供了物质基础.
목적통과NMR정성연구대황소적결구이급대황소결구중적활발성부위,위대황소적화학결구수식제공의거.방법종호장중고효제취료은곤류화합물대황소,채용1D화2D-NMR적분석방법대대황소결구진행감정,통과NMR변온기술급NOESY기술연구활발성부위.결과이DMSO위용제、TMS작내표,채용~1HNMR,~(13)CNMR,DEPF,~1H-~1H COSY,NOESY,HSQC,HMBC등다충핵자공진방법대대황소경보、탄보진행완정귀속,병통과NMR변온기술급NOESY적연구학정료기결구적활발성부위.결론대황소적3위간기위기활발성부위,해신식위대황소적결구수식이급연생물적설계여항종류활성연구제공료물질기출.
OBJECTIVE In order to develop novel anti - tumor agents by modify the structure of Emodin, Studies on the extraction of Emodin and the analysis of structure by NMR were conducted.METHOD The process of extracting Emodin from Polyonum cupida-tum Sieb. Et Zucc was optimized and some changes have been developed with improved overall yield. The structure of Emodin was analyzed by NMR 1D and 2D spectra. By changing temperature technique and NOSEY technique in NMR, the active site of Emodin structure was investigated. RESULT The Proton and 13Carbon spectra of Emodin were assigned completely by 1HNMR, 13CNMR, DEPT, COSY , HSQC, HMBC and NOESY methods. The active site of Emodin structure was verified by changing temperature technique and NOSEY technique in NMR, then structure modifications were performed. CONCLUSION 3-Hydroxy of Emodin is the active site of structure and this information will supply a material basis for anti - tumor activity research of Emodin derivatives.