CAJ | 학술논문

目的建立兔膝关节软骨损伤动物模型，评估白行研制的Ⅱ型胶原海绵修复关节软骨缺损的效果。方法根据既往方法研制成Ⅱ型胶原，利用胶原的自发荧光特性测试其孔径大小；将材料移植到软骨缺损动物模型上，以随机分组对照的方式进行动物实验。通过MRI联合组织病理学HE、番红O、天狼猩红偏振光染色及新生软骨面积测定、Ⅱ型胶原免疫组化染色观察软骨缺损修复状况。结果 Ⅱ型胶原海绵自发荧光分层扫描显示其孔径为(93.26±38.40) μm，较接近最通软骨生长的孔径。通过横向比较动物实验同一时相点各组MRI对照组织学结果可以看出，Ⅱ型胶原海绵组在6周时首先出现零星、孤立的软骨信号，而番红O染色和免疫组化的结果证实新生的软骨同时具有一定的分泌糖胺多糖和Ⅱ型胶原基质的功能。天狼猩红偏振光测定结果显示，Ⅱ型胶原海绵组新生软骨面积远高于对照组(P＜0.01)。结论通过改进方法提取纯化的Ⅱ型胶原，无论组织病理还是MRI的结果均显示对兔膝关节软骨缺损的修复有显著效果。
목적 건립토슬관절연골손상동물모형，평고백행연제적Ⅱ형효원해면수복관절연골결손적효과。 방법 근거기왕방법연제성Ⅱ형효원，이용효원적자발형광특성측시기공경대소；장재료이식도연골결손동물모형상，이수궤분조대조적방식진행동물실험。통과MRI연합조직병이학HE、번홍O、천랑성홍편진광염색급신생연골면적측정、Ⅱ형효원면역조화염색관찰연골결손수복상황。 결과 Ⅱ형효원해면자발형광분층소묘현시기공경위(93.26±38.40) μm，교접근최통연골생장적공경。통과횡향비교동물실험동일시상점각조MRI대조조직학결과가이간출，Ⅱ형효원해면조재6주시수선출현령성、고립적연골신호，이번홍O염색화면역조화적결과증실신생적연골동시구유일정적분비당알다당화Ⅱ형효원기질적공능。천랑성홍편진광측정결과현시，Ⅱ형효원해면조신생연골면적원고우대조조(P＜0.01)。 결론 통과개진방법제취순화적Ⅱ형효원，무론조직병리환시MRI적결과균현시대토슬관절연골결손적수복유현저효과。
Objective To establish rabbit knee joint cartilage injury models to evaluate effects of the type Ⅱ collagen sponge in repair of the articular cartilage. Methods The type Ⅱ collagen sponge was prepared according to previous method and the pore size of the sponges was measured based on the collagen autofluorescence characteristics.The type Ⅱ collagen sponge was transplanted into the injury lesions of the animal model for experimental study.The regeneration of the cartilage defects was observed by using MRI, histologic HE staining, Safranin O, sirius red polarized light staining, areas determination of the newly grown cartilage and immunohistochemistry of type Ⅱ collagen. Results Autofluorescent images of confocal microscope layer scanning showed that the pore size was (93.26 + 38.40) μm in diameter, suitable for chondrocyte growth.Comparison between MRI and H&E staining results showed quicker effusion absorption in the treatment groups than that in the control group, while the level of inflammatory response in the treatment group was lower than that in the control group.The sporadic cartilage signals first appeared at the 6th week.The newly formed cartilage with the expression of glycosaminoglycan and type Ⅱ collagen matrix was confirmed by Safranin O staining and immunohistochemical analysis.The sirius red polarized light staining showed that areas of the newly formed cartilage were significantly larger in the treatment group than that in the control group (P ＜ 0.01). Conclusion The type Ⅱ collagen sponge developed from purification can effectively repair the damaged cartilage tissues of the rabbit knee joints, as has been verified either by MRI or histology.