中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
9期
1074-1075
,共2页
依托咪酯%缺血预处理%细胞凋亡%细胞半胱氨酸天冬氨酸蛋白酶
依託咪酯%缺血預處理%細胞凋亡%細胞半胱氨痠天鼕氨痠蛋白酶
의탁미지%결혈예처리%세포조망%세포반광안산천동안산단백매
Etomidate%Ischemic preconditioning%Apoptosis%Caspases
目的 探讨依托咪酯预处理对HL-60细胞procaspase-3、caspase-9 p35和caspase-8 p20表达的影响.方法 体外培养HL-60细胞,选择指数生长期的细胞,随机分为3组(n=3):对照组(C组)、依托咪酯组(E组)和依托咪酯预处理组(EP组).C组不给予任何处理;E组加入依托咪酯标准品500 μmol/L孵育24 h;EP组先加入依托咪酯标准品1μmol/L孵育1 h,冲洗后于培养基中培养4 h,再加入500 μmol/L依托咪酯孵育24 h.采用Western blot法测定procaspase-3、caspase-9 p35和caspase-8 p20的表达水平.结果 与C组比较,E组和EP组HL-60细胞procaspase-3表达下调,caspase-8 p20和caspase-9 p35表达上调(P＜0.05);与E组比较,EP组HL-60细胞procaspase-3表达上调,caspase-8 p20 和caspase-9 p35表达下调(P＜0.05).结论 依托咪酯预处理可抑制依托咪酯导致的procaspase-3表达下调和caspase-8 p20、caspase-9 p35表达上调,从而抑制依托咪酯诱发的HL-60细胞凋亡.
目的 探討依託咪酯預處理對HL-60細胞procaspase-3、caspase-9 p35和caspase-8 p20錶達的影響.方法 體外培養HL-60細胞,選擇指數生長期的細胞,隨機分為3組(n=3):對照組(C組)、依託咪酯組(E組)和依託咪酯預處理組(EP組).C組不給予任何處理;E組加入依託咪酯標準品500 μmol/L孵育24 h;EP組先加入依託咪酯標準品1μmol/L孵育1 h,遲洗後于培養基中培養4 h,再加入500 μmol/L依託咪酯孵育24 h.採用Western blot法測定procaspase-3、caspase-9 p35和caspase-8 p20的錶達水平.結果 與C組比較,E組和EP組HL-60細胞procaspase-3錶達下調,caspase-8 p20和caspase-9 p35錶達上調(P＜0.05);與E組比較,EP組HL-60細胞procaspase-3錶達上調,caspase-8 p20 和caspase-9 p35錶達下調(P＜0.05).結論 依託咪酯預處理可抑製依託咪酯導緻的procaspase-3錶達下調和caspase-8 p20、caspase-9 p35錶達上調,從而抑製依託咪酯誘髮的HL-60細胞凋亡.
목적 탐토의탁미지예처리대HL-60세포procaspase-3、caspase-9 p35화caspase-8 p20표체적영향.방법 체외배양HL-60세포,선택지수생장기적세포,수궤분위3조(n=3):대조조(C조)、의탁미지조(E조)화의탁미지예처리조(EP조).C조불급여임하처리;E조가입의탁미지표준품500 μmol/L부육24 h;EP조선가입의탁미지표준품1μmol/L부육1 h,충세후우배양기중배양4 h,재가입500 μmol/L의탁미지부육24 h.채용Western blot법측정procaspase-3、caspase-9 p35화caspase-8 p20적표체수평.결과 여C조비교,E조화EP조HL-60세포procaspase-3표체하조,caspase-8 p20화caspase-9 p35표체상조(P＜0.05);여E조비교,EP조HL-60세포procaspase-3표체상조,caspase-8 p20 화caspase-9 p35표체하조(P＜0.05).결론 의탁미지예처리가억제의탁미지도치적procaspase-3표체하조화caspase-8 p20、caspase-9 p35표체상조,종이억제의탁미지유발적HL-60세포조망.
Objective To investigate the effects of etomidate preconditioning on the expression of procaspase-3, caspase-9 p35 and caspase-8 p20 in HL-60 cells. Methods HL-60 cells were purchased from Shanghai life science institute and cultured in RPMI-1640 culture medium at 37℃ in 5% CO2 incubator. The cells were randomy divided into 3 groups ( n = 3 each): control group (group C), etomidate group (group E) and etomidate preconditioning group (group EP). In group E, the cells were exposed to 500 μmol/L etomidate and incubated for 24 h. In group EP, the cells were exposed to 1μmol/L etomidate for 1 h and was allowed to recover for4 h after etomidate washout, then etomidate 500μmol/L was added and the cells were incubated for 24 h. The expression of procaspase-3, caspase-9 p35 and caspase-8 p20 was determined using Western blot. Results The procaspase-3 expression was significantly down-regulated, while the expression of caspase-9 p35 and caspase-8 p20 was up-regulated ingroup E and EP as compared with group C ( P ＜ 0.05). The procaspase-3 expression was up-regulated,while the expression of caspase-9 p35 and caspase-8 p20 was down-regulated in group EP as compared with group E ( P ＜ 0.05). Conclusion Etomidate preconditiong can inhibit etomidate-induced down-regulation of procaspase3 expression and up-regulation of caspase-9 p35 and caspase-8 p20 expression, resulting in suppression of HL-60 cell apoptosis induced by etomidate.
