国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
2期
137-139
,共3页
参芪五味子片%高效液相色谱%蒸发光散射%黄芪甲苷
參芪五味子片%高效液相色譜%蒸髮光散射%黃芪甲苷
삼기오미자편%고효액상색보%증발광산사%황기갑감
Shenqi-wuwcizitablet%HPLC%ELSD%Astragaloside
目的 建立参芪五味子片中黄芪甲苷的含量测定方法.方法 采用高效液相色谱-蒸发光散射(HPLC-ELSD)法,色谱柱Agilent-C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(30:70),流速1.0 ml/min,蒸发管温度90℃,雾化器温度50℃,柱温35℃.结果 黄芪甲苷含量测定的线性范围为41.68～521 μg/ml,回归方程为lnA=1.5461lnC+5.9564,r=0.9995(n=6),平均回收率为100.42%,RSD=1.74%.结论 该方法可作为参芪五味子片质量控制的辅助方法.
目的 建立參芪五味子片中黃芪甲苷的含量測定方法.方法 採用高效液相色譜-蒸髮光散射(HPLC-ELSD)法,色譜柱Agilent-C18柱(150 mm×4.6 mm,5μm),流動相為乙腈-水(30:70),流速1.0 ml/min,蒸髮管溫度90℃,霧化器溫度50℃,柱溫35℃.結果 黃芪甲苷含量測定的線性範圍為41.68～521 μg/ml,迴歸方程為lnA=1.5461lnC+5.9564,r=0.9995(n=6),平均迴收率為100.42%,RSD=1.74%.結論 該方法可作為參芪五味子片質量控製的輔助方法.
목적 건립삼기오미자편중황기갑감적함량측정방법.방법 채용고효액상색보-증발광산사(HPLC-ELSD)법,색보주Agilent-C18주(150 mm×4.6 mm,5μm),류동상위을정-수(30:70),류속1.0 ml/min,증발관온도90℃,무화기온도50℃,주온35℃.결과 황기갑감함량측정적선성범위위41.68～521 μg/ml,회귀방정위lnA=1.5461lnC+5.9564,r=0.9995(n=6),평균회수솔위100.42%,RSD=1.74%.결론 해방법가작위삼기오미자편질량공제적보조방법.
Objective To establish the determination method of Astragaloside Ⅳ in Shenqi-wuweizi tablet. Methods A high performance liquid chromatography method with evaporate light scattering detection was adopted. The analytes were successfully separated with Agilent-C18 column using acetonitrile and water (30:70) as mobile phase at 1.0ml/min. The parameter of ELSD was set at a evaporation tube temperature of 90℃ and nebulization at 50℃. The column temperature was set at 35℃. Results The linear range of Astragaloside Ⅳ was 41.68～521μg/ml, and the calibration curve was lnA=1.5461lnC+5.9564 with a coefficient of 0.9995 (n=6). The average recovery rate was 100.42%, with a relative standard deviation of 1.74%. Conclusion The method was simple, rapid, sensitive and accurate. Besides, it showed good repeatability as well as specificity, suitable for the quality control of Shenqi-wuweizi tablet.
