CAJ | 학술논문

目的研究大鼠异体肢体移植术后急性排斥反应阶段,移植肢体血管内皮细胞细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)动态变化及环孢素A(cyclosporine A,CsA)抗免疫排斥的作用.方法建立大鼠肢体移植动物模型,随机分为对照组(Wistar大鼠→Wistar大鼠)、排斥组(SD大鼠→ Wistar大鼠)和CsA治疗组(SD大鼠→Wistar大鼠),术后1、4、7 d获取移植肢体股动脉行病理学观察,采用免疫组化法检测移植肢体血管ICAM-1表达的变化.结果对照组供体移植肢体股动脉血管内皮细胞仅出现轻微肿胀与ICAM-1表达微弱;排斥组血管内皮细胞肿胀明显,淋巴细胞大量浸润,ICAM-1的表达强度和数量明显增加;CsA治疗组移植肢体血管内皮细胞仅有少量淋巴细胞浸润,ICAM-1表达较弱.结论大鼠异体肢体移植术后急性排斥反应阶段,血管内皮细胞ICAM-1表达水平与排斥反应的发生和发展有关,CsA可降低移植肢体血管内皮细胞ICAM-1表达,抑制复合组织移植术后急性排斥反应.
목적 연구대서이체지체이식술후급성배척반응계단,이식지체혈관내피세포세포간점부분자-1(intercellular adhesion molecule-1,ICAM-1)동태변화급배포소A(cyclosporine A,CsA)항면역배척적작용.방법 건립대서지체이식동물모형,수궤분위대조조(Wistar대서→Wistar대서)、배척조(SD대서→ Wistar대서)화CsA치료조(SD대서→Wistar대서),술후1、4、7 d획취이식지체고동맥행병이학관찰,채용면역조화법검측이식지체혈관ICAM-1표체적변화.결과 대조조공체이식지체고동맥혈관내피세포부출현경미종창여ICAM-1표체미약;배척조혈관내피세포종창명현,림파세포대량침윤,ICAM-1적표체강도화수량명현증가;CsA치료조이식지체혈관내피세포부유소량림파세포침윤,ICAM-1표체교약.결론 대서이체지체이식술후급성배척반응계단,혈관내피세포ICAM-1표체수평여배척반응적발생화발전유관,CsA가강저이식지체혈관내피세포ICAM-1표체,억제복합조직이식술후급성배척반응.
Objective To investigate the dynamic changes of intercellular adhesion molecule-1 (ICAM-1) expression in endothelial cells (EC) during hindlimb allograft acute rejection in rats and the inhibitory effect of cyclospofine A (CsA) on the acute rejection. Methods The rat model of hindlimb allograft was developed. The rats were randomly divided into following groups: control group (Wistar→Wistar), rejection group ( Sprague-Dawley→Wistar) and CsA-treated group (Sprague-Dawley→Wistar). At postoperative day 1, day 4 and day 7samples of the femoral artery from the allograft limb were harvested to observe the pathologic changes. ICAM-1 expression in EC was quantified using immunohistochemical assay. Results Slight swelling and weak ICAM-1expression of EC were observed in the control group. In the rejection groups, obvious EC swelling and massive lymphocyte infiltration were seen. ICAM-1 expression in EC was significantly stronger and elevated. In CsA-group only mild infiltration of lymphocytes was seen and ICAM-I expression in EC was also much weaker.Conclusion During rat hindlimb allograft acute rejection the expression of ICAM-1 in EC was closely related to the occurrence and development of acute rejection. CsA could reduce the expression of ICAM-1 in EC and hence inhibit acute rejection of composite allograft.