CAJ | 학술논문

目的分析中国大陆人感染H5N1高致病性禽流感病例标本采集和病毒分离培养之间的关系.方法对2005年10月至2009年3月中国大陆人感染H5N1高致病性禽流感病例标本进行病毒分离培养.结果标本采集时间主要集中在发病后的0～14 d,其中0～7 d采样的标本阳性率分布相对集中,病毒分离的效果要更加显著;标本采集类型主要为呼吸道标本,其中下呼吸道标本的病毒分离阳性率分布较集中,病毒分离效果也更明显.结论人禽流感病例标本采集时间及标本的采集类型对病毒分离有一定的影响,应在发病的急性期侧重于采集下呼吸道标本,这样利于提高病毒分离的阳性率.
목적 분석중국대륙인감염H5N1고치병성금류감병례표본채집화병독분리배양지간적관계.방법 대2005년10월지2009년3월중국대륙인감염H5N1고치병성금류감병례표본진행병독분리배양.결과 표본채집시간주요집중재발병후적0～14 d,기중0～7 d채양적표본양성솔분포상대집중,병독분리적효과요경가현저;표본채집류형주요위호흡도표본,기중하호흡도표본적병독분리양성솔분포교집중,병독분리효과야경명현.결론 인금류감병례표본채집시간급표본적채집류형대병독분리유일정적영향,응재발병적급성기측중우채집하호흡도표본,저양리우제고병독분리적양성솔.
Objective To analyse the correlation between the virus isolation and the specimen collection of the H5N1 human hish pathogenic avain influenza cases in Mainland China.Methods The specimens were collected in Mainland China from 2005.10 to 2009.3 and the H5N1 viruses were isolated by passage in embryonated chicken eggs.Results Most specimens were obtained within 14 days after disease onset.For the specimens collected within 7 days,the isolation rate was relatively high and the difference of the positive rate between different years was lower than those specimens collected after 7 days.Most of the samples in our study were collected from the upper or lower respiratory tract with few from blood,feces,et al.The isolation rate of lower respiratory specimens was higher and the difference of the positive rate between different years was relatively lower than those from upper respiratory specimens.Conclusion We suggest that the samples should be collected from lower respiratory tract during the acute phase to get the higher isolation rate.