CAJ | 학술논문

目的分析我国中部地区既往采浆HIV感染者HLA Ⅰ类等位基因多态性及其与病毒载量的相关性.方法应用PCR-SSP(序列特异性引物)技术对106例HIV感染者进行HLA Ⅰ类基因分型,分析HLA Ⅰ类等位基因多态性和单元型与血浆病毒载量的相关性.利用ELISPOT技术检测Gag蛋白特异性的CTL反应,并分析其与血浆病毒载量的相关性.结果携带HLA Ⅰ类等位基因纯合子的感染者具有较高的病毒载量(P=0.0098);HLA-A*30、-B*13、-Cw*06、-Cw*14等位基因及A*30/B*13/Cw*06单元型与低病毒载量相关(P=0.0004,0.0103,0.0058,0.0371和0.0006);携带HLA-A*30/B*13/Cw*06单元型的感染者p2p7p1p6蛋白特异性的CTL反应与病毒载量负相关;携带HLA-Cw*14的感染者p17蛋白特异性的CTL反应与病毒载量负相关.结论我国中部地区既往采浆HIV感染人群HLA-A*30、-B*13、-Cw*06、-Cw*14等位基因及A*30/B*13/Cw*06单元型携带者具有较低的病毒载量,其保护性与Gag蛋白特异性的CTL反应有关.
목적 분석아국중부지구기왕채장HIV감염자HLA Ⅰ류등위기인다태성급기여병독재량적상관성.방법 응용PCR-SSP(서렬특이성인물)기술대106례HIV감염자진행HLA Ⅰ류기인분형,분석HLA Ⅰ류등위기인다태성화단원형여혈장병독재량적상관성.이용ELISPOT기술검측Gag단백특이성적CTL반응,병분석기여혈장병독재량적상관성.결과 휴대HLA Ⅰ류등위기인순합자적감염자구유교고적병독재량(P=0.0098);HLA-A*30、-B*13、-Cw*06、-Cw*14등위기인급A*30/B*13/Cw*06단원형여저병독재량상관(P=0.0004,0.0103,0.0058,0.0371화0.0006);휴대HLA-A*30/B*13/Cw*06단원형적감염자p2p7p1p6단백특이성적CTL반응여병독재량부상관;휴대HLA-Cw*14적감염자p17단백특이성적CTL반응여병독재량부상관.결론 아국중부지구기왕채장HIV감염인군HLA-A*30、-B*13、-Cw*06、-Cw*14등위기인급A*30/B*13/Cw*06단원형휴대자구유교저적병독재량,기보호성여Gag단백특이성적CTL반응유관.
Objective To explore the polymorphism of HLA class I alleles of HIV-infected former plasma donors and to investigate its impact on HIV-1 viral load in central China.Methods 106 subjects chronically infected with HIV-1 were recruited and HLA class I alleles were genotyped with PCR-SSP assay.HLA class I genotypes and haplotypes were determined and their association with plasma viral loads were analyzed.Gag-specific CTL responses were detected by an IFN-λ EUSPOT assay by using overlapping peptides,and their association with plasma viral loads were also analyzed.Results Subjects homozygous at HLA class I locus had higher plasma viral loads(P=0.0098);HLA-A*30,-B*13,-Cw*06,-Cw*14 alleles and HLA-A*30/B*13/Cw*06 haplotype were associated with lower plasma viral loads(P=0.0004,0.0103,0.0058,0.0371 and 0.0006);an inverse correlation between p2p7p1p6-specific CTL responses and viral loads in subjects with HLA-A*30/B*13/Cw*06 haplotype as well as an inverse correlation between p17-specific CTL responses and viral loads in subjects with HLA-Cw*14 allele were observed.Conclusion HLA-A*30,-B*13,-Cw*06,-Cw*14 alleles and HLA-A*30/B*13/Cw*06 haplotype were associated with lower plasma viral loads and Gag-specific CTL responses restricted by these HLA alleles may contribute to the protection.