实验动物科学与管理
實驗動物科學與管理
실험동물과학여관리
LABORATORY ANIMAL SCIENCE AND MANAGEMENT
2003年
z1期
34
,共1页
维帕威·维萨瓦托尔%贾萨达·萨卡尔库%卡莫尔波恩·朗格洛杰金达%堪察那·肯库%拉皮班·因班凯乌%万塔尼·拉塔纳萨克%达朗尼·布里帕克蒂·劳森
維帕威·維薩瓦託爾%賈薩達·薩卡爾庫%卡莫爾波恩·朗格洛傑金達%堪察那·肯庫%拉皮班·因班凱烏%萬塔尼·拉塔納薩剋%達朗尼·佈裏帕剋蒂·勞森
유파위·유살와탁이%가살체·살잡이고%잡막이파은·랑격락걸금체%감찰나·긍고%랍피반·인반개오%만탑니·랍탑납살극%체랑니·포리파극체·로삼
AS-30D%肝癌%细胞系%移植%Sprague Dawley 大白鼠
AS-30D%肝癌%細胞繫%移植%Sprague Dawley 大白鼠
AS-30D%간암%세포계%이식%Sprague Dawley 대백서
Objective Pre-transplant status of AS-30D hepatoma cell line was evaluated in Sprague Dawley rats. Cells from different sources, a) in vivo, from the other transplanted Sprague-Dawley rats, b) in vitro, from cultured cell, and c) cryopreservation, from the liquid nitrogen storage tank, were transplanted into 5 week-old female Sprague-Dawley rats (respectively n = 5, 3, and 5). Each animal received intraperitoneal injection of 0.5 ml cell suspension containing 1.5× 106 cells. The results showed no significant difference among groups, in the quality parameters of cell transplantation,such as fluid volume, cell concentration, total cell, cell viability, and the daily consumption of food and water. However, the cell growth duration following the transplantation was lengthened at least one-half fold or 5 days, when other sources of cells than the in vivo, were transplanted into rat abdominal cavity. Thus, the variety of pre-transplant status of the AS-30D cells would enhance flexibility in cell preparations for the successful transplantation in different time frame.
