CAJ | 학술논문

离子束技术是一种有效的农作物诱变和介导转基因技术,本研究利用离子束介导转化选育到一批有价值的小麦变异材料.本文对变异材料的农艺性状、品质性状、种子醇溶蛋白图谱进行了深入分析.结果表明,变异材料的株高、主茎穗长、千粒重、叶片性状、蛋白质含量、湿面筋含量与对照相比都发生了明显的变化并达显著水平;变异材料种子醇溶蛋白谱带已发生了确切的变化,且某些材料已稳定,还有的材料醇溶蛋白谱带存在多态性,还在分离之中.研究结果表明离子束介导转基因技术可产生丰富的变异、创造新的种质资源,并为该技术在小麦育种上的应用提供理论依据.
리자속기술시일충유효적농작물유변화개도전기인기술,본연구이용리자속개도전화선육도일비유개치적소맥변이재료.본문대변이재료적농예성상、품질성상、충자순용단백도보진행료심입분석.결과표명,변이재료적주고、주경수장、천립중、협편성상、단백질함량、습면근함량여대조상비도발생료명현적변화병체현저수평;변이재료충자순용단백보대이발생료학절적변화,차모사재료이은정,환유적재료순용단백보대존재다태성,환재분리지중.연구결과표명리자속개도전기인기술가산생봉부적변이、창조신적충질자원,병위해기술재소맥육충상적응용제공이론의거.
Ion beam technology is an effective technique in inducing breeding and transformation. Some valuable wheat variation materials were obtained using ion beams. The agronomic traits,quality traits,seed gliadin of these materials were studied. The results show that the plant height,main stem panicle length,grain weight,leaf traits,protein content,and wet gluten content of the variations have obvious changes compared with the control and reach a significant level. Seeds gliadin bands of the variation materials changed exactly compared with the control,bands changes of some variation materials have been stable,and that of other materials exist polymorphism,which show that the materials are not stable. The results show that ion beam biotechnology can generate all kinds of variations and new germplasm resources. The results provide a theoretical basis for application of ion beam biotechnology in wheat.