CAJ | 학술논문

目的:比较~(18)F-FLT与~(18)F-FDG在评估肺腺癌化疗早期反应的敏感性和准确性.材料和方法:选择氟尿嘧啶、阿霉素、顺铂3 种化疗药物,分别与肺腺癌A549细胞共同孵育1、4、24和72 h,测定细胞摄取~(18)F-FLT和~(18)F-FDG的变化,与药敏试验MTT法测定的存活细胞数目和细胞抑制率比较,判断两种示踪剂评估化疗反应的敏感性和准确性. 结果:(1)~(18)F-FDG摄取变化:化疗后1h, 除5-FU组~(18)F-FDG摄取明显增高(120±8%,P<0.01)外,顺铂和阿霉素组基本不变.4h、24h,三组变化也无明显变化.72h,三组均明显降低(35±3%,50±2%,55±4%,P<0.01).(2)~(18)F-FLT摄取变化:3种细胞抑制剂均引起~(18)F-FLT摄取明显变化,但变化趋势不同.5-FU对~(18)F-FLT摄取的影响是双相的,治疗后1 h、4h,摄取增加(145±12%,P<0.01 ;150±14%,P<0.01);24h和72h明显减少(43±4%,60±4%P<0.01).阿霉素和顺铂引起~(18)F-FLT摄取变化趋势基本相同,治疗后1h就观察到~(18)F-FLT摄取迅速减少(70±6%,85±4%,P<0.01),24h摄取几乎全部被抑制(26±2%,15±4%,P<0.01).72h摄取较对照组仍降低(35±1%,30±2%,P<0.01).结论:~(18)F-FLT能否反映化疗反应取决于药物的作用机制,5-FU 治疗后激活了肿瘤 DNA 补救合成途径而使早期摄取增高,相反,顺铂和阿霉素则引起摄取减低. ~(18)F-FDG 摄取变化不明显.
목적:비교~(18)F-FLT여~(18)F-FDG재평고폐선암화료조기반응적민감성화준학성.재료화방법:선택불뇨밀정、아매소、순박3 충화료약물,분별여폐선암A549세포공동부육1、4、24화72 h,측정세포섭취~(18)F-FLT화~(18)F-FDG적변화,여약민시험MTT법측정적존활세포수목화세포억제솔비교,판단량충시종제평고화료반응적민감성화준학성. 결과:(1)~(18)F-FDG섭취변화:화료후1h, 제5-FU조~(18)F-FDG섭취명현증고(120±8%,P<0.01)외,순박화아매소조기본불변.4h、24h,삼조변화야무명현변화.72h,삼조균명현강저(35±3%,50±2%,55±4%,P<0.01).(2)~(18)F-FLT섭취변화:3충세포억제제균인기~(18)F-FLT섭취명현변화,단변화추세불동.5-FU대~(18)F-FLT섭취적영향시쌍상적,치료후1 h、4h,섭취증가(145±12%,P<0.01 ;150±14%,P<0.01);24h화72h명현감소(43±4%,60±4%P<0.01).아매소화순박인기~(18)F-FLT섭취변화추세기본상동,치료후1h취관찰도~(18)F-FLT섭취신속감소(70±6%,85±4%,P<0.01),24h섭취궤호전부피억제(26±2%,15±4%,P<0.01).72h섭취교대조조잉강저(35±1%,30±2%,P<0.01).결론:~(18)F-FLT능부반영화료반응취결우약물적작용궤제,5-FU 치료후격활료종류 DNA 보구합성도경이사조기섭취증고,상반,순박화아매소칙인기섭취감저. ~(18)F-FDG 섭취변화불명현.
Purpose:The efficacy of evaluation of changes of tumoral uptake of 3'-deoxy-3'-[~(18)F] fluorothymidine (FLT) was comparatively analyzed with that of ~(18)F-FDG at early stage after anticancer chemotherapy.Materials and Methods:Cells derived from human lung adenocarcinoma were incubated with cisplatin (CDDP),5-fluorouracil(5-FU),doxorubicin (Dox),for 1,4,24 and 72h.The doses(CD-DP: 67 μM; 5-FU 1,540 μM;MTX: 440 μM;) were determined corresponding to a estimated 10% - 95% proliferation inhibition.The cells were allowed to recover before FLT or FDG being added into the culture media for 60 min.Cell counts,viability,estimated by MTT method,were used to evaluate the cytotoxic effects of chemotherapy.Results: FLT uptake was increased significantly at 1 and 4 h after treatment with 5-FU( 145 ± 12%,150 ± 14%,P <0.01).decreased at 24 h and 72 h.In contrast,FLT accumulation was significantly reduced at cytostatic concentrations of CDDP at different time.The uptake of FDG did not change significantly at early time points after treatment,but decreased at 72 h.Conclusion: The tumor cell uptake of FLT revealed specific changes depending on the auti-cancer drug used at much earlier time than FDG after chemotherpay.