CAJ | 학술논문

本研究以豚鼠作为哺乳动物模型,评价了6株HSNl亚型禽流感病毒(AIV)的复制和水平传播能力.经滴鼻接种后,检测到6株病毒中有5株病毒在豚鼠上呼吸道和下呼吸道复制,病毒滴度为0.8 LogEID_(50)mL～3.5 LogEID_(50)mL(或g),豚鼠在感染后第2d至第10d可排出病毒.另外一株病毒A/duck/GX/22/02则仅能在豚鼠的下呼吸道低水平复制.在传播实验中,6株病毒中只有A/duck/GX/35/01能够在豚鼠间水平传播.上述研究结果表明,豚鼠适用于作为高致病性AIV哺乳动物水平传播的模型,而且H5N1亚型AIV在哺乳动物间的水平传播能力不同.本研究为进一步研究AIV在哺乳动物间水平传播的分子机制奠定了良好的基础.
본연구이돈서작위포유동물모형,평개료6주HSNl아형금류감병독(AIV)적복제화수평전파능력.경적비접충후,검측도6주병독중유5주병독재돈서상호흡도화하호흡도복제,병독적도위0.8 LogEID_(50)mL～3.5 LogEID_(50)mL(혹g),돈서재감염후제2d지제10d가배출병독.령외일주병독A/duck/GX/22/02칙부능재돈서적하호흡도저수평복제.재전파실험중,6주병독중지유A/duck/GX/35/01능구재돈서간수평전파.상술연구결과표명,돈서괄용우작위고치병성AIV포유동물수평전파적모형,이차H5N1아형AIV재포유동물간적수평전파능력불동.본연구위진일보연구AIV재포유동물간수평전파적분자궤제전정료량호적기출.
In this study,the replication and transmissibility of 6 H5N1 avian influenza virus strains was evaluated using guinea pig as a mammalian model.Five of the 6 virus strains could be detected in both the upper and lower respiratory tract of guinea pigs from 2 to 10 day alter virus infection.However,the A/duck/GX/22/02 strain could only replicate in the lower respiratory tract.Among tested virus strains,only A/duck/GX/35/01 virus was shown to transmit from guinea pig-to-guinea pig.Our results indicated that guinea pig was a useful mammalian model for the study of influenza virus replication and transmission.