CAJ | 학술논문

目的:建立反相高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定加味西黄软胶囊(牛黄、麝香、蟾酥、乳香、没药)中胆酸的含量;建立气相色谱法测定加味西黄软胶囊中麝香酮的含量.方法:胆酸采用十八烷基键合硅胶色谱柱(Kromasil C_(18),5 μm,4.6 mm×250 mm),流动相为甲醇-0.1%冰醋酸(73:27),流速:1.0 mL/min;EISD检测器参数:蒸发温度40℃,载气(N_2)压力200 kPa.麝香酮的含量测定以正十八烷为内标物,采用DB-1型毛细管色谱柱(30 m×0.32 mm×0.25 μm);FID检测器;程序升温.结果:胆酸在45.2～904 ng范围内呈良好的线性关系,平均回收率为99.06%,RSD为1.56%.麝香酮在0.05～0.5 mg/mL范围内与峰面积比值的线性关系良好,平均回收率为99.40%,RSD为0.95%.结论:本方法简便、准确,可用于该制剂的质量评价.
목적:건립반상고효액상색보-증발광산사검측법(HPLC-ELSD)측정가미서황연효낭(우황、사향、섬소、유향、몰약)중담산적함량;건립기상색보법측정가미서황연효낭중사향동적함량.방법:담산채용십팔완기건합규효색보주(Kromasil C_(18),5 μm,4.6 mm×250 mm),류동상위갑순-0.1%빙작산(73:27),류속:1.0 mL/min;EISD검측기삼수:증발온도40℃,재기(N_2)압력200 kPa.사향동적함량측정이정십팔완위내표물,채용DB-1형모세관색보주(30 m×0.32 mm×0.25 μm);FID검측기;정서승온.결과:담산재45.2～904 ng범위내정량호적선성관계,평균회수솔위99.06%,RSD위1.56%.사향동재0.05～0.5 mg/mL범위내여봉면적비치적선성관계량호,평균회수솔위99.40%,RSD위0.95%.결론:본방법간편、준학,가용우해제제적질량평개.
AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:AC_(18) column(Kromasil C_(18),5 μm,4.6 mm×250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:21)at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40℃,carrier gas(N_2)pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m×0.32 mm×0.25 μm)and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL-0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.