CAJ | 학술논문

目的观察豚鼠哮喘模型中水通道蛋白1(aquapofin 1,AQP)在嗜酸粒细胞(eosinophils,EOS)运动中的作用,探讨AQP1是否可以作为EOS趋化活性的标志.方法选取健康雄性Hartley系豚鼠建立哮喘模型,分别提取外周血及支气管肺泡灌洗液中的EOS,应用原位分子杂交和免疫荧光及激光扫描共聚焦显微镜技术,从基因水平及蛋白质水平检测外周血及支气管肺泡灌洗液中EOS表达AQP1的情况.结果 AQP1在哮喘豚鼠外周血和支气管肺泡灌洗液的EOS内均有表达,外周血中AQP1 mRNA(平均灰度值92.904±3.290,-x±s,下同)和蛋白(荧光强度425.081±17.474)的表达强度均高于灌洗液中AQP1 mRNA(109.200±5.756)和蛋白(279.926±11.293)的表达强度,差异具有统计学意义(t值分别为9.519和27.020,P值均<0.05).结论变应性疾病状态下,EOS可能通过合成更多的AQP1来加速其向炎性靶器官的运动,AQP1有望成为EOS趋化活性的标志之一.
목적 관찰돈서효천모형중수통도단백1(aquapofin 1,AQP)재기산립세포(eosinophils,EOS)운동중적작용,탐토AQP1시부가이작위EOS추화활성적표지.방법 선취건강웅성Hartley계돈서건립효천모형,분별제취외주혈급지기관폐포관세액중적EOS,응용원위분자잡교화면역형광급격광소묘공취초현미경기술,종기인수평급단백질수평검측외주혈급지기관폐포관세액중EOS표체AQP1적정황.결과 AQP1재효천돈서외주혈화지기관폐포관세액적EOS내균유표체,외주혈중AQP1 mRNA(평균회도치92.904±3.290,-x±s,하동)화단백(형광강도425.081±17.474)적표체강도균고우관세액중AQP1 mRNA(109.200±5.756)화단백(279.926±11.293)적표체강도,차이구유통계학의의(t치분별위9.519화27.020,P치균<0.05).결론 변응성질병상태하,EOS가능통과합성경다적AQP1래가속기향염성파기관적운동,AQP1유망성위EOS추화활성적표지지일.
Objective To investigate the role of aquapofin 1(AQP1)in he migration of eosinophils (EOS)and to determine if AQP-1 can be viewed as the chemotactic activity marker of EOS.Methods Asthma model of guinea pigs were developed and EOS were purified from both peripheral blood and bronchoalveolar lavage fluid(BALF).The smears of EOS were studied by in situ hybridization for determining AQP1 mRNA and immunofluorescence under laser scanning confocal microscope for determining AQP1 protein.Results AQP1 was found expressed in EOS both from peripheral blood and from BALF.Compared with the expression of AQP1 mRNA(mean grey value 109.200+-5.756,-x±s)and protein (average fluorescence intensity 279.926+-11.293)in EOS from BALF,there was stronger expression of AQP1 mRNA(92.904±3.290)and protein(425.081±17.474)in EOS from peripheral blood.The difference both of AQP1 mRNA(t=9.519,P<0.05)and protein(t=27.020,P<0.05)were considered statistically significant respectively.Conclusions AQP1 plays a crucial role in EOS movement.It is possible that EOS produce more AQP1 protein to accelerate its migration to inflammatory tiusse under allergic disease and EOS with AQP1 highly expressed are activated.AQP1 can be viewed as the chemotactic activity marker of EOS.