中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
9期
1305-1307
,共3页
史涛坪%马鑫%傅斌%李宏召%艾星%徐华%居正华%王超%张国玺%王保军%张旭
史濤坪%馬鑫%傅斌%李宏召%艾星%徐華%居正華%王超%張國璽%王保軍%張旭
사도평%마흠%부빈%리굉소%애성%서화%거정화%왕초%장국새%왕보군%장욱
膀胱癌%信号转导
膀胱癌%信號轉導
방광암%신호전도
Bladder carcinoma%Signal transduction
目的 探讨Notch信号通路在膀胱移行细胞癌中的表达及与预后间的关系.方法 收集膀胱移行细胞癌组织新鲜标本70例,10例正常膀胱黏膜作为对照组.采用免疫组织化学、逆转录-聚合酶链反应(RT-PCR)和Western blot检测Notch-1、Notch-2、Notch-3、Jagged-1和DLL-1的表达.对70例患者进行随访,结合各基因表达分析其与术后生存时间的关系.结果 在10例正常膀胱移行上皮中以上5种Notch信号通路基因均为深染.非浸润性膀胱癌组织中染色主要集中于细胞膜上,染色较淡,浸润性膀胱癌中染色部位分布于细胞质中,呈片状浓聚.RT-PCR和Western blot发现5种因子在三种组织中的表达差异有统计学意义(P＜0.05).非浸润性膀胱癌患者的术后无瘤生存时间与Notch-1和Jagged-1基因的表达状态相关(P＜0.05).结论 在正常膀胱黏膜上皮、非浸润性膀胱癌和浸润性膀胱癌中Notch信号通路基因的表达呈现3种模式.非浸润性膀胱癌中Notch-1和Jagged-1基因的低表达与患者预后不良相关.
目的 探討Notch信號通路在膀胱移行細胞癌中的錶達及與預後間的關繫.方法 收集膀胱移行細胞癌組織新鮮標本70例,10例正常膀胱黏膜作為對照組.採用免疫組織化學、逆轉錄-聚閤酶鏈反應(RT-PCR)和Western blot檢測Notch-1、Notch-2、Notch-3、Jagged-1和DLL-1的錶達.對70例患者進行隨訪,結閤各基因錶達分析其與術後生存時間的關繫.結果 在10例正常膀胱移行上皮中以上5種Notch信號通路基因均為深染.非浸潤性膀胱癌組織中染色主要集中于細胞膜上,染色較淡,浸潤性膀胱癌中染色部位分佈于細胞質中,呈片狀濃聚.RT-PCR和Western blot髮現5種因子在三種組織中的錶達差異有統計學意義(P＜0.05).非浸潤性膀胱癌患者的術後無瘤生存時間與Notch-1和Jagged-1基因的錶達狀態相關(P＜0.05).結論 在正常膀胱黏膜上皮、非浸潤性膀胱癌和浸潤性膀胱癌中Notch信號通路基因的錶達呈現3種模式.非浸潤性膀胱癌中Notch-1和Jagged-1基因的低錶達與患者預後不良相關.
목적 탐토Notch신호통로재방광이행세포암중적표체급여예후간적관계.방법 수집방광이행세포암조직신선표본70례,10례정상방광점막작위대조조.채용면역조직화학、역전록-취합매련반응(RT-PCR)화Western blot검측Notch-1、Notch-2、Notch-3、Jagged-1화DLL-1적표체.대70례환자진행수방,결합각기인표체분석기여술후생존시간적관계.결과 재10례정상방광이행상피중이상5충Notch신호통로기인균위심염.비침윤성방광암조직중염색주요집중우세포막상,염색교담,침윤성방광암중염색부위분포우세포질중,정편상농취.RT-PCR화Western blot발현5충인자재삼충조직중적표체차이유통계학의의(P＜0.05).비침윤성방광암환자적술후무류생존시간여Notch-1화Jagged-1기인적표체상태상관(P＜0.05).결론 재정상방광점막상피、비침윤성방광암화침윤성방광암중Notch신호통로기인적표체정현3충모식.비침윤성방광암중Notch-1화Jagged-1기인적저표체여환자예후불량상관.
Objective To investigate the expression of Notch receptors and ligands in normal bladder transitional epithelium and transitional cell carcinoma of the bladder and its clinicopathological implications. Methods The expression of Notch-1, Notch-2, Notch-3, Jagged-1 and Delta-like-1 was detected respectively in 70 cases of bladder carcinoma, 10 cases of normal urothelia. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to assay the expression level of Notch factors. The predictive values of the expression for prognosis were investigated by Kaplan-Meier curve. Results All of the 5 kinds of Notch factors were intensively stained in normal bladder transitional epithelia immunohistochemically, but their expression was significantly decreased in tumor tissues. Moreover, the expression level of these 5 genes in the papillary tumors was lower than that in the invasive tumors. Postoperative disease-free survival time of patients with low expression of both Notch-1 and Jagged-1 was significantly shorter than that of patients with other expression patterns in papillary tumors (P ＜0. 05, respectively). Conclusion The expression pattern of Notch family in the papillary TCCB is different from that in invasive TCCB. Low-expression of both Notch-1 and Jagged-1 is potentially a useful marker for survival of patients with papillary TCCB.
