CAJ | 학술논문

背景:有关高血压与一氧化氮关系的研究主要为单一时间的对照研究,但随着血压的改变一氧化氮如何变化的研究较少.目的:观察自发性高血压大鼠(spontaneouly hypertensive rats,SHR)血浆一氧化氮浓度的变化,探讨一氧化氮与高血压发生发展的关系,为判断高血压的预后提供理论依据.设计:非随机对照的实验研究.地点、材料和干预:实验在广州暨南大学完成.选择SHR 30只,雌性6只,雄性9只(高血压组);大鼠WKY 30只,雌性7只,雄性8只(对照组).分别饲养3,6和12个月,测定血压,用硝酸银还原法测定两组大鼠血浆中一氧化氮的含量.主要观察指标:观察随着高血压的不断发展,自发性高血压大鼠血浆一氧化氮浓度的变化.结果:①血压变化:各时期对照组大鼠血压差异无显著性意义,介于(135.0±9.8)～(147.8±11.3)mmHg;高血压组大鼠血压随着月龄增加逐渐升高,12个月达(197.3±7.5)mmHg,各时间点均明显高于对照组大鼠(t=4.132～7.921,P＜0.01).②血浆一氧化氮浓度的变化:对照组大鼠血浆一氧化氮浓度各时间点差异无显著性意义(P＞0.05),维持在(11.5±3.9)～(14.4±3.4)μmol/L水平;高血压组大鼠血浆一氧化氮浓度在3,6个月分别为(16.6±4.5)和(18.5±4.2)μmol/L,明显高于对照组大鼠,但在12个月却明显较对照组大鼠低[(9.3±3.6)μmol/L](t=3.890,P＜0.01),同组内与3,6个月比较亦显著减少(t=3.890,P＜0.01).结论:在高血压发生和发展过程中血浆一氧化氮存在反应性升高的变化,而在高血压的后期,一氧化氮的合成明显不足,这种变化可能与高血压的预后有关.
배경:유관고혈압여일양화담관계적연구주요위단일시간적대조연구,단수착혈압적개변일양화담여하변화적연구교소.목적:관찰자발성고혈압대서(spontaneouly hypertensive rats,SHR)혈장일양화담농도적변화,탐토일양화담여고혈압발생발전적관계,위판단고혈압적예후제공이론의거.설계:비수궤대조적실험연구.지점、재료화간예:실험재엄주기남대학완성.선택SHR 30지,자성6지,웅성9지(고혈압조);대서WKY 30지,자성7지,웅성8지(대조조).분별사양3,6화12개월,측정혈압,용초산은환원법측정량조대서혈장중일양화담적함량.주요관찰지표:관찰수착고혈압적불단발전,자발성고혈압대서혈장일양화담농도적변화.결과:①혈압변화:각시기대조조대서혈압차이무현저성의의,개우(135.0±9.8)～(147.8±11.3)mmHg;고혈압조대서혈압수착월령증가축점승고,12개월체(197.3±7.5)mmHg,각시간점균명현고우대조조대서(t=4.132～7.921,P＜0.01).②혈장일양화담농도적변화:대조조대서혈장일양화담농도각시간점차이무현저성의의(P＞0.05),유지재(11.5±3.9)～(14.4±3.4)μmol/L수평;고혈압조대서혈장일양화담농도재3,6개월분별위(16.6±4.5)화(18.5±4.2)μmol/L,명현고우대조조대서,단재12개월각명현교대조조대서저[(9.3±3.6)μmol/L](t=3.890,P＜0.01),동조내여3,6개월비교역현저감소(t=3.890,P＜0.01).결론:재고혈압발생화발전과정중혈장일양화담존재반응성승고적변화,이재고혈압적후기,일양화담적합성명현불족,저충변화가능여고혈압적예후유관.
BACKGROUND: Most controlled studies on the relationship between hypertension and nitric oxide(NO) examine only the changes of NO at a certain time point, whereas NO changes in relation to the variation of the blood pressure in the development of hypertension are scarcely addressed in randomized studies.OBJECTIVE: To study the changes of plasma NO concentration in spontaneously hypertensive rats(SHR) to understand the relationship between NO and hypertension.DESIGN: Nonrandomized, matched control experiment.SETTING, PARTICIPANTS and INTERVENTION: The experiment was completed in Jinan University. After fed for 3, 6 and 12 months respectively, 15 SHRs(6 females, 9 males) and 15 Wistar-Kyoto(WKY) rats(7 females, 8males) were sacrificed to measure the blood pressure and determined plasma NO levels with the silver nitrate deoxidize method.MAIN OUTCOME MEASURES: The change of plasma NO concentration in SHRs with the development of hypertension was observed.RESULTS: The blood pressure of WKY rats varied insignificantly between (135.0 ±9.8) and(147.8 ± 11.3) mmHg in the course of 1-year observation, but in SHRs, significantly elevated blood pressure was observed in comparison with the WKY rats( t =4. 132-7.921, P ＜ 0.01), reaching the height of(197.3 ±7. 5) mmHg in the 12th month. Plasma NO concentration of WKY rats underwent slight variation between(11. 5 ±3. 9) and(14. 4 ±3.4)μmol/L, but was significantly increased in SHRs at the 3rd and 6th months [(16. 6 ±4. 5) μ mol/L and(18. 5 ±4. 2) μ mol/L] then lowered to(9.3 ±3.6) μ mol/L, which was below the level in WKY rats at the 12th months( t =3.890, P ＜ 0. 01) and those at the 3rd and 6th months in SHRs( t = 3. 890, P ＜0.01 ). There was a positive correlation between plasma NO concentration and the blood pressure at 3 and 6 months, but an inverse one in the 12th month.CONCLUSION: Plasma NO concentration is elevated in response to the onset and progression of hypertension, but in advanced stage of hypertension,NO synthesis is apparently insufficient, which may be indicative of the prognosis of hypertension.