CAJ | 학술논문

目的探讨一种可以替代脑脊液细胞手工计数法实现标准化操作的可行性方法 .方法应用iQ200工作站对100例脑脊液患者进行白细胞和红细胞的检测,并与显微镜计数板法相比较.结果细胞检测范围在0～3 200个/微升时,两种方法比较差异无统计学意义(P>0.05),在3 30012 000个/微升细胞时比较差异有统计学意义(P<0.05);两种方法均有较高的精密度,iQ200工作站法略高于计数板法;计数结果呈高度相关,红细胞相关系数r=0.998 5,白细胞相关系数r=0.999 3;iQ200工作站还可以通过影像分析技术对细胞进行回顾性甄别.结论 iQ200工作站在一定范围内用于脑脊液细胞计数有良好的精密度和准确性,适用于脑脊液细胞计数,可达到检测的标准化和规范化.
목적 탐토일충가이체대뇌척액세포수공계수법실현표준화조작적가행성방법 .방법 응용iQ200공작참대100례뇌척액환자진행백세포화홍세포적검측,병여현미경계수판법상비교.결과 세포검측범위재0～3 200개/미승시,량충방법 비교차이무통계학의의(P>0.05),재3 30012 000개/미승세포시비교차이유통계학의의(P<0.05);량충방법 균유교고적정밀도,iQ200공작참법략고우계수판법;계수결과 정고도상관,홍세포상관계수r=0.998 5,백세포상관계수r=0.999 3;iQ200공작참환가이통과영상분석기술대세포진행회고성견별.결론 iQ200공작참재일정범위내용우뇌척액세포계수유량호적정밀도화준학성,괄용우뇌척액세포계수,가체도검측적표준화화규범화.
Objective To establish a standardized detecting method for cerebrospinal fluid cells which replaces the manual method. Methods The iQ200 workstation was applied to measuring WBC and RBC in 100 cases of cerebrospinal fluid,and the test results were compared with those measured with microscopic counting plate assay. Results There were no statistical differences in the test results in the detecting range 0-3 200/μL between the two detecting methods (P>0.05),but there were in the range 3 300 - 12 000/μL (P< 0.05). Both methods showed high precision, and precision of the iQ200 workstation was higher than that of manual method. The results were closely correlated with each other (r=0. 998 5 for RBC counting,0. 999 3 for WBC counting). The iQ200 workstation can ret-rospectively discriminate hematocytes by applying image analysis technology. Conclusion It is accurate and reliable to detect and categorize the cerebrospinal fluid cells with the iQ200 workstation. The standardization can be achieved by using the iQ200 workstation.