CAJ | 학술논문

采用DALP分子标记技术,对10个川百合居群进行DNA指纹检测,结果筛选出5个引物组合,扩增后共产生192条DNA基因片段,其中178条谱带具有遗传多态性,占92.71%;10个居群的总等位基凶数(Na)为1.9271、平均为1.0823,总有效等位基冈数(Ne)为1.4430、平均1.0533,基因多样性指数(H)为0.2673、平均0.0303,总Shannon多样性指数(I)为0.4105、平均为0.0447,基因分化系数(Gst)为0.8874.即遗传变异有88.74%发生在居群间、有11.26%发生在居群内,表明川百合居群间存在较大的遗传分化.此外,昆明市西山的川百合野生居群(DC)的遗传多样性明显高于栽培居群,这与川百合栽培品种长期人工驯化有直接关系.同时野生居群受到人为活动干扰严重.研究结果表明,DALP分析可为川百合遗传育种的可持续利用提供理论依据.
채용DALP분자표기기술,대10개천백합거군진행DNA지문검측,결과사선출5개인물조합,확증후공산생192조DNA기인편단,기중178조보대구유유전다태성,점92.71%;10개거군적총등위기흉수(Na)위1.9271、평균위1.0823,총유효등위기강수(Ne)위1.4430、평균1.0533,기인다양성지수(H)위0.2673、평균0.0303,총Shannon다양성지수(I)위0.4105、평균위0.0447,기인분화계수(Gst)위0.8874.즉유전변이유88.74%발생재거군간、유11.26%발생재거군내,표명천백합거군간존재교대적유전분화.차외,곤명시서산적천백합야생거군(DC)적유전다양성명현고우재배거군,저여천백합재배품충장기인공순화유직접관계.동시야생거군수도인위활동간우엄중.연구결과표명,DALP분석가위천백합유전육충적가지속이용제공이론의거.
DALP(Direct amplification of length polymorphism)was applied to detect genetic polymorphism of 10 populations of Lilium davidii Duchartre.Five primer groups were screened, and a total of 192 DNA fragments were amplified, among which 178 were polymorphic, i.e. the percentage of polymorphic bands (PPB) was 92.71%. The total observed number of alleles (Na)wasl.9271 and the average Na was1.0823; the total effective number of alleles (Ne) was 1.4430 and the average Ne was 1.0533;the total Nei's gene diversity(H) was 0.2673 and the average H wasO.0303; the total Shannon's Information index (I) was 0.4105 and the average I was 0.0447. The coefficient of gene differentiation (Gst) was 0.8874 between populations, namely 88.74% genetic variation occurring between populations and rest 11.26% within population,the result indicated that L davidii had much larger genetic differentiation among populations.The genetic diversity of population in Xishan Kunming (DC)was evidently higher than that planting populations, this was directly related to the long acclimation of L davidii by artificial culture, and wild populations were severely interfered by human activities. DALP analysis provides this species with molecule evidence for the sustainable exploitation of genetic breeding.