上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
6期
681-684
,共4页
申锷%张跃力%刁雪红%胡兵
申鍔%張躍力%刁雪紅%鬍兵
신악%장약력%조설홍%호병
Apocynin%糖尿病%心肌肥大%心肌纤维化%心脏功能
Apocynin%糖尿病%心肌肥大%心肌纖維化%心髒功能
Apocynin%당뇨병%심기비대%심기섬유화%심장공능
Apocynin%diabetes%cardiac hypertrophy%cardiac fibrosis%cardiac function
目的 探讨NADPH氧化酶选择性抑制剂Apocynin对1型糖尿病小鼠心脏功能的影响及其作用机制.方法 44只8周龄雄性C57小鼠随机分为正常对照组(n=6)、Apocynin对照组(n=6)、1型糖尿病组(n=16)及1型糖尿病Apocynin治疗组(n=16).小鼠腹腔注射链脲佐菌素(STZ)建立1型糖尿病动物模型.实验于8周末结束,记录实验小鼠生存率、测量小鼠体质量及左室质量,并计算左室质量指数.运用超声心动图技术与Langendorff灌注系统检测小鼠心脏功能;Real-time RT-PCR技术测定心肌组织α-肌球蛋白重链(α-MHC)、β-肌球蛋白重链(β-MHC)、心房钠尿肽(ANP)以及Ⅰ型(Col Ⅰ)和Ⅲ型(Col Ⅲ)胶原的表达.结果 ①Apocynin能提高糖尿病小鼠生存率,降低糖尿病小鼠左室质量指数(P<0.01);②Apocynin能明显改善糖尿病小鼠心脏功能,增加心脏左室射血分数、左室短轴缩短率和+dp/dt、-dp/dt(P<0.01);③Apocynin能显著降低糖尿病小鼠心肌ANP mRNA表达和β/α-MHC比值(P<0.01);④Apocynin能降低糖尿病小鼠心肌组织Col Ⅰ和Col Ⅲ胶原的表达(P<0.01).结论 Apocynin能显著改善1型糖尿病小鼠的心脏功能,其机制与降低心肌肥大特征性基因ANP表达和β/α-MHC比值以及减少心肌Col Ⅰ、Col Ⅲ胶原表达密切相关.
目的 探討NADPH氧化酶選擇性抑製劑Apocynin對1型糖尿病小鼠心髒功能的影響及其作用機製.方法 44隻8週齡雄性C57小鼠隨機分為正常對照組(n=6)、Apocynin對照組(n=6)、1型糖尿病組(n=16)及1型糖尿病Apocynin治療組(n=16).小鼠腹腔註射鏈脲佐菌素(STZ)建立1型糖尿病動物模型.實驗于8週末結束,記錄實驗小鼠生存率、測量小鼠體質量及左室質量,併計算左室質量指數.運用超聲心動圖技術與Langendorff灌註繫統檢測小鼠心髒功能;Real-time RT-PCR技術測定心肌組織α-肌毬蛋白重鏈(α-MHC)、β-肌毬蛋白重鏈(β-MHC)、心房鈉尿肽(ANP)以及Ⅰ型(Col Ⅰ)和Ⅲ型(Col Ⅲ)膠原的錶達.結果 ①Apocynin能提高糖尿病小鼠生存率,降低糖尿病小鼠左室質量指數(P<0.01);②Apocynin能明顯改善糖尿病小鼠心髒功能,增加心髒左室射血分數、左室短軸縮短率和+dp/dt、-dp/dt(P<0.01);③Apocynin能顯著降低糖尿病小鼠心肌ANP mRNA錶達和β/α-MHC比值(P<0.01);④Apocynin能降低糖尿病小鼠心肌組織Col Ⅰ和Col Ⅲ膠原的錶達(P<0.01).結論 Apocynin能顯著改善1型糖尿病小鼠的心髒功能,其機製與降低心肌肥大特徵性基因ANP錶達和β/α-MHC比值以及減少心肌Col Ⅰ、Col Ⅲ膠原錶達密切相關.
목적 탐토NADPH양화매선택성억제제Apocynin대1형당뇨병소서심장공능적영향급기작용궤제.방법 44지8주령웅성C57소서수궤분위정상대조조(n=6)、Apocynin대조조(n=6)、1형당뇨병조(n=16)급1형당뇨병Apocynin치료조(n=16).소서복강주사련뇨좌균소(STZ)건립1형당뇨병동물모형.실험우8주말결속,기록실험소서생존솔、측량소서체질량급좌실질량,병계산좌실질량지수.운용초성심동도기술여Langendorff관주계통검측소서심장공능;Real-time RT-PCR기술측정심기조직α-기구단백중련(α-MHC)、β-기구단백중련(β-MHC)、심방납뇨태(ANP)이급Ⅰ형(Col Ⅰ)화Ⅲ형(Col Ⅲ)효원적표체.결과 ①Apocynin능제고당뇨병소서생존솔,강저당뇨병소서좌실질량지수(P<0.01);②Apocynin능명현개선당뇨병소서심장공능,증가심장좌실사혈분수、좌실단축축단솔화+dp/dt、-dp/dt(P<0.01);③Apocynin능현저강저당뇨병소서심기ANP mRNA표체화β/α-MHC비치(P<0.01);④Apocynin능강저당뇨병소서심기조직Col Ⅰ화Col Ⅲ효원적표체(P<0.01).결론 Apocynin능현저개선1형당뇨병소서적심장공능,기궤제여강저심기비대특정성기인ANP표체화β/α-MHC비치이급감소심기Col Ⅰ、Col Ⅲ효원표체밀절상관.
Objective To investigate the effects of Apocynin, the NADPH oxidase inhibitor, on cardiac function in type 1 diabetic mice and explore the mechanisms. Methods Forty-four 8-week-old C57 mice were randomly divided into control group (n=6), Apocynin control group(n=6), type 1 diabetes group (n=16) and type 1 diabetes treated by Apocynin group (n=16). Type 1 diabetes mouse models were induced by STZ injection. Eight weeks after model establishment, the survival rate, body weight (BW), left ventricular heart weight (LVHW) and LVHW/BW were measured, the cardiac function was analysed by ultrasound cardiography and Langendorff perfusion system, and the expression of α-myosin heavy chain (α-MHC), β-myosin heavy chain (β-MHC), atrial natriuretic peptide (ANP), type Ⅰ collagen(Col Ⅰ)and type Ⅲ collagen(Col Ⅲ)of cardiac muscles was detedcted by Real-time RT-PCR.Results ①Apocynin significantly increased the survival rate and decreased LVHW/BW in diabetic mice(P<0.01).②Apocynin significantly improved the cardiac function, increased left ventricular ejection function, left ventricular short axis fractional shortening, +dp/dt and-dp/dt in diabetic mice(P<0.01).③ Apocynin significantly decreased the ecpression of ANP mPNA and ratios of β/α-MHC of cardiac muscles in diabetic mice (P < 0.01).④ Apocynin significantly decrease the expression of Col Iand Col Ⅲ of cardiac muscles in diabetic mice (P < 0.01). Conclusion Apocynin significantly improves the cardiac function of type 1 diabetic mice, which is associated with the decreased expression of hypertrophy-related gene ANP, β/α-MHC ratio and expression of Col Ⅰ and Col Ⅲ in cardiac muscles.
