CAJ | 학술논문

目的研究A族β溶血性链球菌感染菌(GAS)株emm分型及其超抗原在我国儿童中的分布情况.方法用聚合酶链反应(PCR)联合测序对2005年～2006年中国5家儿童医院的222株A族β溶血性链球菌感染的临床菌株进行emm分型;PCR检测speA,speC,speH,speI,speG,speJ,ssa和SMEZ超抗原基因的分布情况.结果共检测到9个emm型,emm12为主占55.86%(124/222),其次emm1占39.64%(88/222);78.39%的A族溶血性链球菌(GAS)分离株携带有6个或以上的超抗原基因,emm型和超抗原基因谱之间存在密切联系.结论中国儿童GAS感染流行菌株具有emm型分布集中和超抗原基因携带率高的特点,不同的emm分型有不同的超抗原基因谱,这将为我国GAS疫苗的研制提供重要的分子流行病学依据.
목적 연구A족β용혈성련구균감염균(GAS)주emm분형급기초항원재아국인동중적분포정황.방법 용취합매련반응(PCR)연합측서대2005년～2006년중국5가인동의원적222주A족β용혈성련구균감염적림상균주진행emm분형;PCR검측speA,speC,speH,speI,speG,speJ,ssa화SMEZ초항원기인적분포정황.결과 공검측도9개emm형,emm12위주점55.86%(124/222),기차emm1점39.64%(88/222);78.39%적A족용혈성련구균(GAS)분리주휴대유6개혹이상적초항원기인,emm형화초항원기인보지간존재밀절련계.결론 중국인동GAS감염류행균주구유emm형분포집중화초항원기인휴대솔고적특점,불동적emm분형유불동적초항원기인보,저장위아국GAS역묘적연제제공중요적분자류행병학의거.
Objective To investigate the distribution of the emm types and superantigens of group A streptococcal ( GAS) isolated from Chinese children. Methods Totally 222 GAS isolates collected from five Children's Hospitals of China during 2005～2006 were studied, emm types were performed by PCR and sequencing. The eight superan-tigen (SAg) genes (speA, speC, speH, speI, speG, speJ, ssa and SMEZ) were checked by PCR. Results Nine emm types were identified, of which emml2 (55. 86% ) and emml (39. 64% ) were the most prevalent types. The GAS isolates carried six or more SAg genes take 78. 39% of all the isolates in this study. The SAg gene profiles were closely associated with the emm type. Conclusion The emm type of S. pyogenes isolated from Chinese chil-dren was quite wide-spreading and SAg genes appeared to be associated with the emm type so its expression is po-tential in vaccine development.