CAJ | 학술논문

为考察溶析结晶纯化去乙酰真菌环氧乙酯的效果,研究了不同的溶剂、反溶剂含量和导入方式对去乙酰真菌环氧乙酯结晶的影响.在静态结晶实验的基础上,通过设计正交实验L_9(3~4)研究动态结晶过程中各因素对去乙酰真菌环氧乙酯收率和纯度的影响.结果表明:有效地控制反溶剂含量及其导入方式可以提高晶体的纯度和收率并影响晶体的形态.去乙酰真菌环氧乙酯动态结晶最佳条件:结晶温度为15 ℃,溶剂-反溶剂体积比(丙酮-水)为2∶3,反溶剂流加时间为3 h.
위고찰용석결정순화거을선진균배양을지적효과,연구료불동적용제、반용제함량화도입방식대거을선진균배양을지결정적영향.재정태결정실험적기출상,통과설계정교실험L_9(3~4)연구동태결정과정중각인소대거을선진균배양을지수솔화순도적영향.결과표명:유효지공제반용제함량급기도입방식가이제고정체적순도화수솔병영향정체적형태.거을선진균배양을지동태결정최가조건:결정온도위15 ℃,용제-반용제체적비(병동-수)위2∶3,반용제류가시간위3 h.
In order to purify deacetylmycoepoxydiene(DAM)using solventing-out crystallization technique,the optimal conditions of crystallization were investigated by orthogonal design,and the recovery and purity of DAM were used as major evaluating contents.The results showed that the shapes of DAM crystal were influenced by different solvents,the recovery and purity of DAM were also influenced by the volume of anti-solvent and the way it was conducted.The optimal parameters are described as follows:crystallization temperature is 15 ℃, V (acetone)∶ V (water)=2∶3,the flowing time of anti-solvent is 3 hours.