CAJ | 학술논문

[目的]采用RAPD法研究孝感荸荠和野生荸荠的遗传差异性.[方法]利用随机扩增多态性DNA(RAPD)技术,对地方栽培种孝感荸荠、野生荸荠、蒲草和扁杆藨草4种植物的基因组DNA进行了遗传差异性分析.[结果]筛选出841、842、807和840共4个随机引物,其中841随机引物的扩增产物多态性明显,获得清晰重复性好的条带.聚类结果显示栽培荸荠和野生荸荠的亲缘性相对于蒲草和鹿茸杆藨草而言较近.[结论]该研究为培植优质的荸荠新品种提供了理论基础.
[목적]채용RAPD법연구효감발제화야생발제적유전차이성.[방법]이용수궤확증다태성DNA(RAPD)기술,대지방재배충효감발제、야생발제、포초화편간표초4충식물적기인조DNA진행료유전차이성분석.[결과]사선출841、842、807화840공4개수궤인물,기중841수궤인물적확증산물다태성명현,획득청석중복성호적조대.취류결과현시재배발제화야생발제적친연성상대우포초화록용간표초이언교근.[결론]해연구위배식우질적발제신품충제공료이론기출.
[Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA (RAPD) technology. [Method] Genetic diversities of the local cultivated water chestnut,wild chestnut,Lepironia articulata and Scirpus planiculmis Fr . Schmidt were analyzed by RAPD technology. [Result] Among the screened random primers 841,842,807 and 840,the polymorphism of amplification product of 841 was evident,and the obtained bands in electrophoresis were clear and showed good repeatability. Cluster analysis result showed that the affinity of cultivated water chestnut and wild water chestnut was nearer than that between Lepironia articulata and Scirpus planiculmis. [Conclusion] The research provides theoretical basis for cultivating high-quality new varieties of water chestnut.