CAJ | 학술논문

背景:对于组织工程血管而言,如何在平滑肌细胞层上成功获得致密的内皮细胞层是最为关键的.目的:探索不同细胞种植浓度对构建全生物化组织工程血管的影响.方法:先将不同浓度(5×105,5×107 L-1)猪血管平滑肌细胞种植在猪脱细胞血管基质上,培养3 d后再将不同浓度(5×105,5×107 L-1)内皮祖细胞接种在平滑肌细胞-血管基质复合体上,构建片状全生物化组织工程材料.结果与结论:高浓度与低浓度平滑肌细胞在脱细胞血管基质上的细胞生长曲线相似,并且种植在孔板上和在脱细胞基质上的生长曲线亦相似,但低浓度组增殖较慢,覆盖率较低.细胞覆盖率由高到低的顺序为:高浓度内皮祖细胞+含高浓度平滑肌细胞的脱细胞基质>高浓度内皮祖细胞+含低浓度平滑肌细胞的脱细胞基质>低浓度内皮祖细胞+含高浓度平滑肌细胞的脱细胞基质>低浓度内皮祖细胞+含低浓度平滑肌细胞的脱细胞基质,且高浓度内皮祖细胞在脱细胞基质上可形成较为致密的细胞层,呈现出铺路石样生长方式.说明提高细胞接种浓度有利于其在材料表面快速形成致密的细胞层.
배경:대우조직공정혈관이언,여하재평활기세포층상성공획득치밀적내피세포층시최위관건적.목적:탐색불동세포충식농도대구건전생물화조직공정혈관적영향.방법:선장불동농도(5×105,5×107 L-1)저혈관평활기세포충식재저탈세포혈관기질상,배양3 d후재장불동농도(5×105,5×107 L-1)내피조세포접충재평활기세포-혈관기질복합체상,구건편상전생물화조직공정재료.결과여결론:고농도여저농도평활기세포재탈세포혈관기질상적세포생장곡선상사,병차충식재공판상화재탈세포기질상적생장곡선역상사,단저농도조증식교만,복개솔교저.세포복개솔유고도저적순서위:고농도내피조세포+함고농도평활기세포적탈세포기질>고농도내피조세포+함저농도평활기세포적탈세포기질>저농도내피조세포+함고농도평활기세포적탈세포기질>저농도내피조세포+함저농도평활기세포적탈세포기질,차고농도내피조세포재탈세포기질상가형성교위치밀적세포층,정현출포로석양생장방식.설명제고세포접충농도유리우기재재료표면쾌속형성치밀적세포층.
BACKGROUND: How to successfully obtain compact endothelium layers on smooth muscle cells is the most crucial part for the tissue-engineered vessels. OBJECTIVE: To explore the effects of different cell implantation concentrations on the construction of the complete biological tissue-engineered blood vessels.METHODS: Different concentrations of porcine vascular smooth muscle cells (5×105, 5×107 cells/L) were implanted on the porcine acellular vascular matrix to culture for 3 days. Then different concentrations of endothelial progenitor cells (5×105, 5×107 cells/L) were implanted on the smooth muscle cell-vascular matrix composite to construct lamellar complete biological tissue-engineered blood vessels.RESULTS AND CONCLUSION: The growth curves of high concentrations of smooth muscle cells on the acellular vascular matrix were similar to that of low concentrations. Moreover, the growth curves of cells implanted in the culture plates were similar to that implanted on the acellular matrix. However, cells in the low concentration groups have relatively low proliferation activity and low coverage rate. The cell coverage rate decreased as follows: high concentrations of endothelial progenitor cells+acellular matrix containing high concentrations of smooth muscle cells > high concentrations of endothelial progenitor cells+acellular matrix containing low concentrations of smooth muscle cells > low concentrations of endothelial progenitor cells+acellular matrix containing high concentrations of smooth muscle cells > low concentrations of endothelial progenitor cells+acellular matrix containing low concentrations of smooth muscle cells. Moreover, high concentrations of endothelial progenitor cells form relatively compact layers on the acellular matrix and show cobble-like growth. These findings indicate that an increase in the cell implantation concentrations is beneficial to the rapid formation of compact cell layers on the material surface.