中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2011年
12期
805-809
,共5页
乳腺肿瘤%蛋白质酪氨酸激酶%血管内皮生长因子C%NF-κB%淋巴转移
乳腺腫瘤%蛋白質酪氨痠激酶%血管內皮生長因子C%NF-κB%淋巴轉移
유선종류%단백질락안산격매%혈관내피생장인자C%NF-κB%림파전이
Breast neoplasm%Protein-tyrosine kinase%Vascular endothelial growth factor C%NF-kappa B%Lynphatic metastasis
目的 研究Syk基因对乳腺癌血管内皮生长因子(VEGF)-C表达的调控机制.方法 采用免疫组织化学(EnVision法)检测乳腺癌组织中Syk、NFκB与VEGF-C的蛋白表达情况,分析三者的相关性及与淋巴转移的关系.转染pcDNA3.1(-)-Syk至乳腺癌MDA-MB-231细胞,检测对VEGF-C和NFκB表达的影响.结果 在淋巴转移组中,Syk蛋白阳性率低于非淋巴转移组,VEGF-C与NFκB阳性率在淋巴转移组高于非淋巴转移组.Syk蛋白表达与NFκB(r=-0.448,P=0.002)、VEGF-C(r=-0.620,P=0.000)蛋白表达呈负相关,VEGF-C与NFκB呈正相关(r =0.310,P=0.036).转染pcDNA3.1(-)-Syk重组质粒的乳腺癌细胞中VEGF-C的mRNA及蛋白表达水平均低于空白对照组,细胞核NFκB蛋白表达低于空白对照组(均P＜0.05).结论 Syk基因与乳腺癌转移相关,可能是通过抑制NFκB的活性而下调VEGF-C的表达,从而抑制乳腺癌的淋巴转移.
目的 研究Syk基因對乳腺癌血管內皮生長因子(VEGF)-C錶達的調控機製.方法 採用免疫組織化學(EnVision法)檢測乳腺癌組織中Syk、NFκB與VEGF-C的蛋白錶達情況,分析三者的相關性及與淋巴轉移的關繫.轉染pcDNA3.1(-)-Syk至乳腺癌MDA-MB-231細胞,檢測對VEGF-C和NFκB錶達的影響.結果 在淋巴轉移組中,Syk蛋白暘性率低于非淋巴轉移組,VEGF-C與NFκB暘性率在淋巴轉移組高于非淋巴轉移組.Syk蛋白錶達與NFκB(r=-0.448,P=0.002)、VEGF-C(r=-0.620,P=0.000)蛋白錶達呈負相關,VEGF-C與NFκB呈正相關(r =0.310,P=0.036).轉染pcDNA3.1(-)-Syk重組質粒的乳腺癌細胞中VEGF-C的mRNA及蛋白錶達水平均低于空白對照組,細胞覈NFκB蛋白錶達低于空白對照組(均P＜0.05).結論 Syk基因與乳腺癌轉移相關,可能是通過抑製NFκB的活性而下調VEGF-C的錶達,從而抑製乳腺癌的淋巴轉移.
목적 연구Syk기인대유선암혈관내피생장인자(VEGF)-C표체적조공궤제.방법 채용면역조직화학(EnVision법)검측유선암조직중Syk、NFκB여VEGF-C적단백표체정황,분석삼자적상관성급여림파전이적관계.전염pcDNA3.1(-)-Syk지유선암MDA-MB-231세포,검측대VEGF-C화NFκB표체적영향.결과 재림파전이조중,Syk단백양성솔저우비림파전이조,VEGF-C여NFκB양성솔재림파전이조고우비림파전이조.Syk단백표체여NFκB(r=-0.448,P=0.002)、VEGF-C(r=-0.620,P=0.000)단백표체정부상관,VEGF-C여NFκB정정상관(r =0.310,P=0.036).전염pcDNA3.1(-)-Syk중조질립적유선암세포중VEGF-C적mRNA급단백표체수평균저우공백대조조,세포핵NFκB단백표체저우공백대조조(균P＜0.05).결론 Syk기인여유선암전이상관,가능시통과억제NFκB적활성이하조VEGF-C적표체,종이억제유선암적림파전이.
Objective To investigate the effect of Syk on the VEGF-C expression in breast cancer.Methods Immunohistochemical EnVision method was used to detect the protein expression of Syk,NFκBand VEGF-C in breast carcinoma; and the relationship between protein expression of Syk,NFκB,VEGF-C and lymph node metastasis was analysed.MDA-MB-231 cells were transfected with pcDNA3.1 ( - )-Syk,and the effect of Syk gene on the VEGF-C and NFκB expression was determined.Results In the lymph node metastatic group,a lower expression rate of Syk and higher expression rate of VEGF-C and NFκB were detected as compared to the non-metastatic group.The expression of Syk was negatively associated with NFκB (r=-0.448,P=0.002) and VEGF-C (r=-0.620,P=0.000) expression,and VEGF-C was associated with the nuclear expression of NFκB ( r =0.310,P =0.036).Compared with the non-transfected cells,the pcDNA3.1 ( -)-Syk transfected MDA-MB-231 cells showed significantly lower transcriptional level of VEGF-C mRNA,expression level of VEGF-C protein and NFκB activity ( P ＜ 0.05 ).Conclusions Syk may play an important role in the lymph node metastasis of breast cancer.It may down-regulate the expression of VEGF-C by inhibiting the activity of NFκB,which thus suppresses lymph node metastasis of breast cancer.
