CAJ | 학술논문

目的探讨γδT细胞在系统性红斑狼疮(SLE)患者外周血中的变化及临床意义.方法流式细胞术检测42例SLE患者、20名健康对照组外周血中CD3+γδ+T细胞百分率及绝对数；用膜联蛋白-Ⅴ/碘化丙啶法检测6例活动期SLE患者及6名正常对照组外周血中CD3+T细胞及γδ+T细胞的凋亡率.酶联免疫吸附试验( ELISA)法测定42例SLE患者血清抗核抗体和抗双链DNA(dsDNA)抗体水平.统计学方法采用t检验及Pearson相关分析.结果活动期SLE患者外周血中T细胞百分率[(3.0±1.8)％]较非活动期[(5.3±3.0)％]及健康对照组[(6.8±2.8)％]均显著下降(t值分别为-3.071和-5.913,P均＜o.01)；活动期SLE患者γδT细胞绝对数[(1.7±1.6)×107/L]明显低于非活动期[(5.3±3.6) ×107/L](t=-3.292,P＜0.01),且两者均低于健康对照组[(10.1 ±5.0) ×107/L](t值分别为-7.247和-2.905,P均＜0.01).活动期SLE患者γδT细胞绝对数与SLE疾病活动指数(SLEDAI)评分呈负相关(r=-0.365,P=0.047).SLE患者外周血中γδT细胞百分率(r=-0.336,P=0.030)及绝对数(r=0.410,P=0.007)均与红细胞沉降率( ESR)呈负相关,γδT细胞绝对数与血红蛋白呈正相关(r=0.409,P=0.007).此外,SLE患者外周血中γδT细胞凋亡率显著高于健康对照组及SLE患者CD3+T细胞凋亡率(t值分别为2.886和2.952,P均＜0.05).结论 SLE患者外周血中γδT细胞数量下降,与病情活动相关；γδT细胞数量下降的机制可能与凋亡增加有关.
목적 탐토γδT세포재계통성홍반랑창(SLE)환자외주혈중적변화급림상의의.방법 류식세포술검측42례SLE환자、20명건강대조조외주혈중CD3+γδ+T세포백분솔급절대수；용막련단백-Ⅴ/전화병정법검측6례활동기SLE환자급6명정상대조조외주혈중CD3+T세포급γδ+T세포적조망솔.매련면역흡부시험( ELISA)법측정42례SLE환자혈청항핵항체화항쌍련DNA(dsDNA)항체수평.통계학방법채용t검험급Pearson상관분석.결과 활동기SLE환자외주혈중T세포백분솔[(3.0±1.8)％]교비활동기[(5.3±3.0)％]급건강대조조[(6.8±2.8)％]균현저하강(t치분별위-3.071화-5.913,P균＜o.01)；활동기SLE환자γδT세포절대수[(1.7±1.6)×107/L]명현저우비활동기[(5.3±3.6) ×107/L](t=-3.292,P＜0.01),차량자균저우건강대조조[(10.1 ±5.0) ×107/L](t치분별위-7.247화-2.905,P균＜0.01).활동기SLE환자γδT세포절대수여SLE질병활동지수(SLEDAI)평분정부상관(r=-0.365,P=0.047).SLE환자외주혈중γδT세포백분솔(r=-0.336,P=0.030)급절대수(r=0.410,P=0.007)균여홍세포침강솔( ESR)정부상관,γδT세포절대수여혈홍단백정정상관(r=0.409,P=0.007).차외,SLE환자외주혈중γδT세포조망솔현저고우건강대조조급SLE환자CD3+T세포조망솔(t치분별위2.886화2.952,P균＜0.05).결론 SLE환자외주혈중γδT세포수량하강,여병정활동상관；γδT세포수량하강적궤제가능여조망증가유관.
Objective To investigate the number of γδT cells in the peripheral blood from patients with systemic lupus erythematosus (SLE) and their correlation to disease activity.Methods γδT cells were detected in the peripheral blood from 42 SLE patients and 20 normal controls by flow cytometry.Anniex-V/Pl double-staining flow cytometry was employed to observe the proportion of the apoptotic γδ and CD3+ T cells in 6 SLE patients with active disease and 6 normal controls,respectively.The levels of plasma anti-nuclear antibody and anti-dsDNA antibody were determined by using enzyme-linked immunosorbent assay.Data were analyzed with t test and Pearson's correlation test.Results The percentages of γδT cells were remarkably down-regulated in SLE patients [(3.0±1.8)％ ] with active disease compared with that of those patients with inactive [(5.3±3.0)％] disease and normal controls [(6.8±2.8)％](t=-3.071 and -5.913 respectively,both P＜0.01 ).The absolute number of γδT cells decreased significantly in SLE patients with active disease [ ( 1.7± 1.6)× 107/L ] than those with inactive SLE [ (5.3±3.6)× 107/L ] (t=-3.292,P＜0.01 ),and both were lower than the normal controls [ (10.1±5.0)×l 07/L] (t=-7.247 and -2.905 respectively,both P＜0.01 ).There was a negative correlation between systemic lupus erythematosus disease activity index (SLEDAI) and T cell counts in 30 SLE patients with active disease (r=-0.365,P=0.047).γδT cell percentage (r=-0.336,P=0.030) and counts (r=-0.410,P=0.007) were both inversely correlated with the erythrocyte sedimentation rate,but positive correlation were found between hemoglobulln and γδT cell counts (r=0.409,P=0.007).The apoptosis of γδT cells in SLE patients was more common than in normal controls (t=2.886,P＜0.05 ).The number of apoptotic γδT cells was higher than that of CD3+ T cells in SLE patients (t=2.952,P＜0.05 ).Conclusion γδT cells of the peripheral blood of SLE patients are down-regulated partially due to excessive apoptosis,which may correlate with the disease activity.