中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
5期
600-603
,共4页
麻醉药,吸入%心肌再灌注损伤%胱硫醚β合酶%血红素加氧酶-1
痳醉藥,吸入%心肌再灌註損傷%胱硫醚β閤酶%血紅素加氧酶-1
마취약,흡입%심기재관주손상%광류미β합매%혈홍소가양매-1
Anesthetics,inhalation%Myocardial repeffusion injury%Cystathionine beta-synthase%Heme oxygenase-1
目的 评价七氟醚预先给药对大鼠心肌缺血再灌注损伤时胱硫醚β-合酶(CBS)和血红素氧合酶-1(HO-1)表达的影响.方法 健康成年雄性SD大鼠30只,体重180～220 g,采用随机数字表法,将大鼠随机分为3组(n=10):假手术组(S组)、缺血再灌注损伤组(I/R组)和七氟醚组(Sev组).结扎左冠状动脉前降支,缺血30 min,再灌注2h,制备心肌缺血再灌注损伤模型.Sev组于缺血前吸入七氟醚,呼气末浓度1.5％～1.7％,60 min后制备心肌缺血再灌注损伤模型.于再灌注2h时处死大鼠,取心肌组织,测定MDA含量(硫代巴比妥酸法)、SOD活性(黄嘌呤氧化酶法)、GSH含量(荧光法)、H2S含量(分光光度法)、CO含量(分光光度法)、CBS mRNA和HO-1 mRNA表达(RT-PCR法),电镜下观察心肌细胞超微结构.结果 与S组比较,I/R组和Sev组CO、H2S、MDA含量和心肌细胞线粒体变性率升高,CBS mRNA和HO-1 mRNA表达上调,SOD活性及GSH含量降低(P＜0.05)；与I/R组比较,Sev组CO、H2S、MDA含量和心肌细胞线粒体变性率降低,CBS mRNA和HO-1 mRNA表达下调,SOD活性和GSH含量升高(P＜0.05).结论 七氟醚预先给药减轻心肌缺血再灌注损伤的机制与下调CBS和HO-1的表达有关.
目的 評價七氟醚預先給藥對大鼠心肌缺血再灌註損傷時胱硫醚β-閤酶(CBS)和血紅素氧閤酶-1(HO-1)錶達的影響.方法 健康成年雄性SD大鼠30隻,體重180～220 g,採用隨機數字錶法,將大鼠隨機分為3組(n=10):假手術組(S組)、缺血再灌註損傷組(I/R組)和七氟醚組(Sev組).結扎左冠狀動脈前降支,缺血30 min,再灌註2h,製備心肌缺血再灌註損傷模型.Sev組于缺血前吸入七氟醚,呼氣末濃度1.5％～1.7％,60 min後製備心肌缺血再灌註損傷模型.于再灌註2h時處死大鼠,取心肌組織,測定MDA含量(硫代巴比妥痠法)、SOD活性(黃嘌呤氧化酶法)、GSH含量(熒光法)、H2S含量(分光光度法)、CO含量(分光光度法)、CBS mRNA和HO-1 mRNA錶達(RT-PCR法),電鏡下觀察心肌細胞超微結構.結果 與S組比較,I/R組和Sev組CO、H2S、MDA含量和心肌細胞線粒體變性率升高,CBS mRNA和HO-1 mRNA錶達上調,SOD活性及GSH含量降低(P＜0.05)；與I/R組比較,Sev組CO、H2S、MDA含量和心肌細胞線粒體變性率降低,CBS mRNA和HO-1 mRNA錶達下調,SOD活性和GSH含量升高(P＜0.05).結論 七氟醚預先給藥減輕心肌缺血再灌註損傷的機製與下調CBS和HO-1的錶達有關.
목적 평개칠불미예선급약대대서심기결혈재관주손상시광류미β-합매(CBS)화혈홍소양합매-1(HO-1)표체적영향.방법 건강성년웅성SD대서30지,체중180～220 g,채용수궤수자표법,장대서수궤분위3조(n=10):가수술조(S조)、결혈재관주손상조(I/R조)화칠불미조(Sev조).결찰좌관상동맥전강지,결혈30 min,재관주2h,제비심기결혈재관주손상모형.Sev조우결혈전흡입칠불미,호기말농도1.5％～1.7％,60 min후제비심기결혈재관주손상모형.우재관주2h시처사대서,취심기조직,측정MDA함량(류대파비타산법)、SOD활성(황표령양화매법)、GSH함량(형광법)、H2S함량(분광광도법)、CO함량(분광광도법)、CBS mRNA화HO-1 mRNA표체(RT-PCR법),전경하관찰심기세포초미결구.결과 여S조비교,I/R조화Sev조CO、H2S、MDA함량화심기세포선립체변성솔승고,CBS mRNA화HO-1 mRNA표체상조,SOD활성급GSH함량강저(P＜0.05)；여I/R조비교,Sev조CO、H2S、MDA함량화심기세포선립체변성솔강저,CBS mRNA화HO-1 mRNA표체하조,SOD활성화GSH함량승고(P＜0.05).결론 칠불미예선급약감경심기결혈재관주손상적궤제여하조CBS화HO-1적표체유관.
Objective To investigate the effect of sevoflurane pretreatment on the expression of cystathionine β-synthase(CBS)and heme oxygerase-1(HO-1)during myocardial ischemia-reperfusion(I/R)injury in rats.Methods Thirty adult male SD rats,weighing 180-220 g,were randomly divided into 3 groups(n =10each):sham operation group(group S),I/R group and sevoflurane group(group Sev).Myocardial I/R injury was produced by ligation of the left anterior descending branch of coronary artery for 30 min followed by 2 h reperfusioo.In group Sev,sevoflurane was inhaled before ischemia,the end-tidal concentration was 1.5％-1.7％ and myocardial I/R was produced 60 min later.The rats were sacrificed at 2 h of reperfusion and myocardial tissues were taken for determination of the contents of MDA,GSH,H2S and CO,SOD activity and expression of CBS mRNA and HO-I mRNA.The ultrastructure of myocardium was examined with electron microscope.Results Compared with group S,the contents of MDA,H2S and CO were significantly increased,the expression of CBS mRNA and HO-1 mRNA was up-regulated,and SOD activity and GSH content were significantly decreased in groups I/R and Sev(P ＜ 0.05).Compared with group I/R,the contents of MDA,H2S and CO were significanfly decreased,the expression of CBS mRNA and HO-1 mRNA was down-regulated,SOD activity and GSH content were significantly increased in group Sev.The damage to mitochondrial structure induced by I/R was mitigated by pretreatment with sevoflurane.Conclusion The mechanism by which sevoflurane pretreatment reduces myocardial I/R injury is related to down-regulation of the expression of CBS and HO-1 and decrease in the activities in rats.
