CAJ | 학술논문

目的越来越多的研究证实,活化的肝星状细胞(hepatic stellate cells,HSCs)具有免疫抑制功能.本研究观察了HSCs对T细胞介导的适应性免疫应答的影响及其与调节性T细胞(regulatory T cells,Treg)的关系.方法分离培养小鼠HSCs,运用混合淋巴细胞反应(mixed lymphocyte reaction,MLR),体外观察活化的HSCs对T细胞增殖和Treg细胞的影响.结果活化的HSCs能导致树突状细胞(dendritic cells,DCs)介导的适应性免疫应答中T细胞的低反应性:抑制T细胞的增殖,同时诱导Treg细胞的表达.结论活化的HSCs能通过促进Treg细胞表达而诱导适应性免疫应答中T细胞的活化无能,促进免疫耐受.
목적 월래월다적연구증실,활화적간성상세포(hepatic stellate cells,HSCs)구유면역억제공능.본연구관찰료HSCs대T세포개도적괄응성면역응답적영향급기여조절성T세포(regulatory T cells,Treg)적관계.방법 분리배양소서HSCs,운용혼합림파세포반응(mixed lymphocyte reaction,MLR),체외관찰활화적HSCs대T세포증식화Treg세포적영향.결과 활화적HSCs능도치수돌상세포(dendritic cells,DCs)개도적괄응성면역응답중T세포적저반응성:억제T세포적증식,동시유도Treg세포적표체.결론 활화적HSCs능통과촉진Treg세포표체이유도괄응성면역응답중T세포적활화무능,촉진면역내수.
Objective Accumulating reports have suggested that hepatic stellate cells (HSCs) exhibit immunosuppressive ability and may be responsible for the occurrence and development of hepatocellular carcinoma (HCC).The mechanisms through which HSCs affect T-cell-induced adaptive immune responses and the relationship with the regulatory T cells (Treg cells) were studied.Methods We isolated HSCs from wildtype mice to demonstrate the influence of HSCs on T-cell proliferation and explored their effect on Treg cells through mixed leukocyte reactions (MLRs) in vitro.Results We found that activated HSCs could induce T-cell hyporesponsiveness in adaptive immune response by inhibiting the proliferation of T cells andincreasing the quantity of Treg cells.Conclusion Activated HSCs may lead to hypoergia of T cells in adaptive immune reaction and up-regulate the expression of Treg cells,thus facilitating immunotolarance.