中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
7期
804-807
,共4页
狄飚%白志军%王玉林%罗雷%陈妤%蒋力云%杨智聪%王鸣
狄飚%白誌軍%王玉林%囉雷%陳妤%蔣力雲%楊智聰%王鳴
적표%백지군%왕옥림%라뢰%진여%장력운%양지총%왕명
登革病毒%E基因%系统进化树
登革病毒%E基因%繫統進化樹
등혁병독%E기인%계통진화수
Dengue virus%E gene%Phylogenetic tree
目的 对广州市2009年新出现的3型登革病毒(DEN)株的E基因进行RT-PCR扩增和序列测定,探讨其来源及基因型.方法 收集广州市2009年登革热患者急性期血清,用C6/36细胞培养分离DEN,RT-PCR扩增病毒全长E基因,测序并绘制系统进化树,结合流行病学资料进行分子流行病学分析.结果 2009年采集的19份患者血清标本中,分离到7株3型DEN株,RT-PCR扩增后测序获得E基因序列,7株DEN3病毒E基因均由1479个碱基组成,编码493个氨基酸,基因序列未见插入或缺失,分析发现7株病毒来自两个不同的亚型:09/GZ/1081、09/GZ/1483和09/GZ/10806属于东南亚/南太平洋型,09/GZ/10616、09/GZ/11144、09/GZ/11194和09/GZ/13105属于印度次大陆型,各亚型群内毒株序列同源性较高.结论 广州市2009年DEN3为输入性,分属两个基因型.
目的 對廣州市2009年新齣現的3型登革病毒(DEN)株的E基因進行RT-PCR擴增和序列測定,探討其來源及基因型.方法 收集廣州市2009年登革熱患者急性期血清,用C6/36細胞培養分離DEN,RT-PCR擴增病毒全長E基因,測序併繪製繫統進化樹,結閤流行病學資料進行分子流行病學分析.結果 2009年採集的19份患者血清標本中,分離到7株3型DEN株,RT-PCR擴增後測序穫得E基因序列,7株DEN3病毒E基因均由1479箇堿基組成,編碼493箇氨基痠,基因序列未見插入或缺失,分析髮現7株病毒來自兩箇不同的亞型:09/GZ/1081、09/GZ/1483和09/GZ/10806屬于東南亞/南太平洋型,09/GZ/10616、09/GZ/11144、09/GZ/11194和09/GZ/13105屬于印度次大陸型,各亞型群內毒株序列同源性較高.結論 廣州市2009年DEN3為輸入性,分屬兩箇基因型.
목적 대엄주시2009년신출현적3형등혁병독(DEN)주적E기인진행RT-PCR확증화서렬측정,탐토기래원급기인형.방법 수집엄주시2009년등혁열환자급성기혈청,용C6/36세포배양분리DEN,RT-PCR확증병독전장E기인,측서병회제계통진화수,결합류행병학자료진행분자류행병학분석.결과 2009년채집적19빈환자혈청표본중,분리도7주3형DEN주,RT-PCR확증후측서획득E기인서렬,7주DEN3병독E기인균유1479개감기조성,편마493개안기산,기인서렬미견삽입혹결실,분석발현7주병독래자량개불동적아형:09/GZ/1081、09/GZ/1483화09/GZ/10806속우동남아/남태평양형,09/GZ/10616、09/GZ/11144、09/GZ/11194화09/GZ/13105속우인도차대륙형,각아형군내독주서렬동원성교고.결론 엄주시2009년DEN3위수입성,분속량개기인형.
Objective To analyze and trace the infection source the envelope(E) gene of the new emerged type 3 dengue virus in Guangzhou in 2009. Methods Sera were collected from patients infected with local dengue fever. Dengue virus was cultured and isolated by C6/36 cells. The whole length E gene was amplified from the positive specimen by RT-PCR, thereby sequenced and phylogenetic tree drawn by neighbor-joining method. Both data on epidemiologic and molecular studies were processed and analysed. Results 7 strains of type 3 dengue virus were isolated from samples of the 19 patients. E gene of these strains was amplified. The complete E genes of 7 strains belonged to 1479 nucleotides in length, encoding a polyprotein of 493 amino acids. Data from the phylogenetic analysis showed that 09/GZ/1081, 09/GZ/1483 and 09/GZ/10806 strains fell within the Southeast Asia/South Pacific group. 09/GZ/10616, 09/GZ/11144, 09/GZ/11194 while 09/GZ/13105 strains fell within the India group. Conclusion The type 3 dengue virus identified in Guangzhou area in 2009 was imported and could be devided into two genotypes.