CAJ | 학술논문

目的研究转化生长因子β1( TGF-p1)基因启动子多态性各等位基因及基因型在肝癌(HCC)患者中的分布频率,初步分析其基因型及血清水平与HCC的相关性.方法采用限制性片段长度多态性聚合酶链反应( PCR-RFLP)技术,检测102例HCC患者和110例健康对照TGF-β1的基因多态性,包括TGF-β1基因启动子-800G/A、-509C/T位点,采用酶联免疫吸附试验(ELISA)检测血清TGF-β1水平.结果 HCC患者TGF-β1水平显著高于对照组[(51.06±9.74) μg/L,(22.12±8.67)μg/L,t=22.884,P＜0.01],TGF-β1基因-800G/A位点多态性在HCC组和健康对照组中的分布差异无统计学意义(P＞0.05),而TGF-β1基因-509C/T多态性各等位基因及基因型频率在两组人群中的分布差异有统计学意义(P＜0.05)；等位基因频率的相对风险分析发现,T等位基因携带者患HCC的风险是C等位基因的1.822倍(OR=1.822,95 ％CI:1.238～2.682,t=22.884,P＜0.01),携带T等位基因的HCC患者血清TGF-β1水平高于不携带者[(53.52±10.07) μg/L,(43.57±9.89)μg/L,t=3.898,P＜ 0.01].结论 TGF-β1基因-509C/T多态性与HCC之间存在相关关系；携带T等位基因的个体可能通过促进TGF-β1的高度表达而增加HCC的发病风险.
목적 연구전화생장인자β1( TGF-p1)기인계동자다태성각등위기인급기인형재간암(HCC)환자중적분포빈솔,초보분석기기인형급혈청수평여HCC적상관성.방법 채용한제성편단장도다태성취합매련반응( PCR-RFLP)기술,검측102례HCC환자화110례건강대조TGF-β1적기인다태성,포괄TGF-β1기인계동자-800G/A、-509C/T위점,채용매련면역흡부시험(ELISA)검측혈청TGF-β1수평.결과 HCC환자TGF-β1수평현저고우대조조[(51.06±9.74) μg/L,(22.12±8.67)μg/L,t=22.884,P＜0.01],TGF-β1기인-800G/A위점다태성재HCC조화건강대조조중적분포차이무통계학의의(P＞0.05),이TGF-β1기인-509C/T다태성각등위기인급기인형빈솔재량조인군중적분포차이유통계학의의(P＜0.05)；등위기인빈솔적상대풍험분석발현,T등위기인휴대자환HCC적풍험시C등위기인적1.822배(OR=1.822,95 ％CI:1.238～2.682,t=22.884,P＜0.01),휴대T등위기인적HCC환자혈청TGF-β1수평고우불휴대자[(53.52±10.07) μg/L,(43.57±9.89)μg/L,t=3.898,P＜ 0.01].결론 TGF-β1기인-509C/T다태성여HCC지간존재상관관계；휴대T등위기인적개체가능통과촉진TGF-β1적고도표체이증가HCC적발병풍험.
Objectives To study the relationship between the allele frequencies and genotype distribution of transforming growth factor-beta 1 (TGF-β1) gene promoter polymorphisms in Chinese patients with heptocellular carcinoma(HCC),and to analyze the association of the serum levels and genotype of TGF-β1 with HCC.Methods The polymorphisms of TGF-β gene,including polymorphisms of TGF-[β1 gene -800G/A、-509C/T,were analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)methods in 102 patients with HCC and 110 healthy controls,and the serum level of TGF-β1 was determined by enzyme-linked immunosorbent assay (ELISA). Results The HCC group showed significantly higher serum levels of TGF-β1 than control group [(51.06 ± 9.74)μg/L,(22.12 ± 8.67 )μg/L,t=22.884, P＜0.01], The distributions of TGF-β gene -800G/A polymorphisms were not different significantly between HCC group and control group, but TGF-β1 -509C/T gene polymorphism was significantly different. The relative risk suffered from HCC of C allele was 1.822 times of the T allele (OR=1.822,95 ％CI:1.238-2.682,t=22.884,P＜0.01), the serum level of TGF-β1 T allele carriers was significantly higher than that of no carriers [(53.52:±:10.07) μg/L,(43.57±9.89)μ.g/L,t=3.898, P＜0.01]. Conclusion TGF-β1 gene -509C/T polymorphism is associated with HCC, and T allele may be a risk factor for HCC, in which the TGF-β1 T allele carriers may have increased risk by enhancing the TGF-β1 expression in the pathogenesis of HCC.