CAJ | 학술논문

[目的]建立一种新的测试范围宽、分辨率高、重现性好的底液，采用电位溶出分析技术，检测人发锌含量。[方法]应用微分电位溶出仪(DPSA-Ⅱ型)，以磺基水杨酸 -KOH底液，选择试验条件，描制标准曲线，并进行样本前处理。[结果]检出限为0.001 μg／mL，线性范围0.02～0.2μg／mL，回收率为96.2％～106.1％，批内CV7.5％，30例人发样本测定结果与原子吸收法对照，两组之间无显著意义(t＜0.05，P＞0.05)。151例健康学龄前儿童发锌测定结果符合我国儿童发锌偏低的报道。[结论]磺基水杨酸 -KOH底液在微分电位溶出法中，应用简便、快捷、重现性好，适用于一般实验室开展发锌检测。
[목적]건립일충신적측시범위관、분변솔고、중현성호적저액，채용전위용출분석기술，검측인발자함량。[방법]응용미분전위용출의(DPSA-Ⅱ형)，이광기수양산 -KOH저액，선택시험조건，묘제표준곡선，병진행양본전처리。[결과]검출한위0.001 μg／mL，선성범위0.02～0.2μg／mL，회수솔위96.2％～106.1％，비내CV7.5％，30례인발양본측정결과여원자흡수법대조，량조지간무현저의의(t＜0.05，P＞0.05)。151례건강학령전인동발자측정결과부합아국인동발자편저적보도。[결론]광기수양산 -KOH저액재미분전위용출법중，응용간편、쾌첩、중현성호，괄용우일반실험실개전발자검측。
[Objective] To develop a new base solution with wide detection range, high resolution capacity andgood repeatibility, for detecting zinc in human hair with potentiometric stripping analysis. [Methocs] The base solu-tion of sulfosalicylic acid-KOH for differential potentiometric stripping analyzer (DPSA-Ⅱ) was applied in this assay. The screening of experimental conditions, preparing standard curve and pretreatment of samples were studied.[Results] The detection limit, linear range, recovery rate and internal coefficient of variation was 0.001μg/mL, 0.02～0.2 μg/mL, 96.2％～106.1％ and 7.5％ respectively. There was no significant differences between the levels ofzinc in 30 human hair samples obtained from this assay and atomic absorption spectrophotometry (t＜0. 05, P＞0.05). The levels of hair zinc of 151 healthy preschool children determined by this method accorded with the reportthat hair zinc of chinese children stayed in lower level. [Con cluslon]Application of base solution of sulfosalicylic acidKOH in differential potentiometric stripping analysis, was easy and rapid to operate, present good repeatibility,andsuitable for detection of human hair zinc levels among common laboratories.