CAJ | 학술논문

Objective: To determine the effective nucleotide sites ofribozymes against HCV ,and obtain a highly effective,nontoxic and inexpensive antisense ribozyme specific for HCV.Methods: Two effective ribozymes,targeted to HCV 5'NC region and C region,were designed and synthesized.Eukaryotic expression vectors,pSV2-gpt.CD-SR (,containing either HCRZNC or HCRZC were constructed and transfected into MT-2 cells,which had been infected by HCV.Quantitative PCR and hybridization were used to determine the effect of inhibition of HCV by ribozymes.Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%,respectively.Furthermore,when both ribozymes were cotransfected into cells,they suppressed replication by 78.8%.Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells,and have better effect when used together.