中国科学C辑(英文版)
中國科學C輯(英文版)
중국과학C집(영문판)
SCIENCE IN CHINA (Series C)
2003年
6期
584-594
,共11页
刘宗旨%王建龙%王群%黄勋%徐卫辉%朱立煌%何平%方荣祥
劉宗旨%王建龍%王群%黃勛%徐衛輝%硃立煌%何平%方榮祥
류종지%왕건룡%왕군%황훈%서위휘%주립황%하평%방영상
Oryza sativa%GRP%gene structure%expression pattern%chromosomal localization
Glycine-rich proteins (GRPs) belong to a kind of important structural proteins of plant cell walls. The expression of GRP genes is regulated spatially and developmentally as well as by various environmental stresses, thus providing a good model for the study of plant gene expression. We obtained the genomic sequence of a new GRP gene (Osgrp-2) from a rice genomic library. The transcription start site of Osgrp-2 was determined by 5'-rapid amplification of cDNA ends (RACE) and a 2.4-kb promoter sequence was thus delimited. The spatial and developmental expression pattern as well as the wound-inducible character of Osgrp-2 in rice plants was analyzed in detail. Furthermore, the gene was mapped onto rice chromosome 10 by analysis of restriction fragment length polymorphism (RFLP).
