胚胎干细胞源性肝干细胞肝内移植对宿主肝功能的影响及安全性评估
배태간세포원성간간세포간내이식대숙주간공능적영향급안전성평고
Effect of intrahepatic transplantation of embryonic stem cells-derived hepatic stem cells on host hepatic function and its safety evaluation
  • 万方数据
    中国组织工程研究与临床康复 중국조직공정연구여림상강복
    JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
    2008年 8期 1591-1595 ,共5页

    邓小耿%宋尔卫%闵军%张杰%陈伦%曾炳胜%方天翎%陈积圣

    산소경%송이위%민군%장걸%진륜%증병성%방천령%진적골

    胚胎干细胞%肝细胞移植%诱导分化%治疗性肝再生%淤胆血清 胚胎榦細胞%肝細胞移植%誘導分化%治療性肝再生%淤膽血清
    배태간세포%간세포이식%유도분화%치료성간재생%어담혈청
    背景:体外诱导胚胎干细胞分化为肝细胞已有不少成功的报道,但其体内移植后能否有效整合入宿主肝板、在肝内能否进一步生长分化并表达肝细胞功能以及成瘤的风险等情况目前还不清楚.目的:应用治疗性肝再生模型进行胚胎干细胞源性肝干细胞肝内移植, 观察其在肝组织替代、体内的生长分化及成瘤性情况.设计:随机对照动物实验.单位:中山大学附属第二医院小儿外科.材料:选用BALB/c小鼠24只为受体,鼠龄6～8周,体质量20～ 35 g,雌雄不拘购自广州市实验动物中心.实验所用胚胎干细胞源性肝干细胞由作者所在课题组诱导胚胎干细胞分化而成.小鼠胚胎干细胞株E14由本院干细胞中心提供.方法:实验于2006-07/2007-06在中山大学附属第二医院干细胞研究中心完成.将24只小鼠随机分为2组:肝再生模型+干细胞移植组和肝切除+干细胞移植组,每组12只.前组分两次按50 mg/kg剂量腹腔内注射倒千里光碱(retrorsine) ,间隔2周,第2次注射4周后行70%肝部分切除制造肝损伤;然后经门静脉分别移植1×105羟基荧光素乙酰乙酸(CFDA-SE)荧光标记的细胞入小鼠肝内进行胚胎干细胞源性肝干细胞移植.后组在行70%肝部分切除制造肝损伤模型后进行胚胎干细胞源性肝干细胞移植.主要观察指标:荧光显微镜下观察移植细胞组受体鼠肝脏内分布、整合与体内生长分化情况.2周后行白蛋白荧光免疫组化(双荧光染色)、血清白蛋白水平检测其功能状况.将胚胎干细胞源性肝干细胞注入治疗性肝再生小鼠肝内,将未分化的胚胎干细胞移植入小鼠腋区皮下作为对照,观察胚胎干细胞源性肝干细胞体内成瘤情况.结果:①肝干细胞在受体鼠肝内生长情况:CFDA SE标记的胚胎干细胞源性肝干细胞肝内移植1周,受体小鼠肝实质内可见散在绿色荧光分布.2周后,肝实质内绿色荧光分布区域明显扩大,且可见类似肝索样结构排列.②肝功能:共焦白蛋白荧光免疫组化(双荧光染色)结果表明,受体小鼠肝组织内可见标记细胞表达白蛋白阳性信号(呈黄色荧光),肝再生模型+干细胞移植组和肝切除+干细胞移植组血清白蛋白水平则无明显差异(P > 0.05).③肝干细胞移植安全性:6周内未见畸胎瘤形成,而将未分化的胚胎干细胞移植入小鼠腋区皮下6周后则可见畸胎瘤形成.结论:胚胎干细胞源性肝干细胞移植入治疗性肝再生模型小鼠肝内后可有效在肝内能进一步生长分化并部分表达肝细胞功能;且此移植安全性较好.
    배경:체외유도배태간세포분화위간세포이유불소성공적보도,단기체내이식후능부유효정합입숙주간판、재간내능부진일보생장분화병표체간세포공능이급성류적풍험등정황목전환불청초.목적:응용치료성간재생모형진행배태간세포원성간간세포간내이식, 관찰기재간조직체대、체내적생장분화급성류성정황.설계:수궤대조동물실험.단위:중산대학부속제이의원소인외과.재료:선용BALB/c소서24지위수체,서령6～8주,체질량20～ 35 g,자웅불구구자엄주시실험동물중심.실험소용배태간세포원성간간세포유작자소재과제조유도배태간세포분화이성.소서배태간세포주E14유본원간세포중심제공.방법:실험우2006-07/2007-06재중산대학부속제이의원간세포연구중심완성.장24지소서수궤분위2조:간재생모형+간세포이식조화간절제+간세포이식조,매조12지.전조분량차안50 mg/kg제량복강내주사도천리광감(retrorsine) ,간격2주,제2차주사4주후행70%간부분절제제조간손상;연후경문정맥분별이식1×105간기형광소을선을산(CFDA-SE)형광표기적세포입소서간내진행배태간세포원성간간세포이식.후조재행70%간부분절제제조간손상모형후진행배태간세포원성간간세포이식.주요관찰지표:형광현미경하관찰이식세포조수체서간장내분포、정합여체내생장분화정황.2주후행백단백형광면역조화(쌍형광염색)、혈청백단백수평검측기공능상황.장배태간세포원성간간세포주입치료성간재생소서간내,장미분화적배태간세포이식입소서액구피하작위대조,관찰배태간세포원성간간세포체내성류정황.결과:①간간세포재수체서간내생장정황:CFDA SE표기적배태간세포원성간간세포간내이식1주,수체소서간실질내가견산재록색형광분포.2주후,간실질내록색형광분포구역명현확대,차가견유사간색양결구배렬.②간공능:공초백단백형광면역조화(쌍형광염색)결과표명,수체소서간조직내가견표기세포표체백단백양성신호(정황색형광),간재생모형+간세포이식조화간절제+간세포이식조혈청백단백수평칙무명현차이(P > 0.05).③간간세포이식안전성:6주내미견기태류형성,이장미분화적배태간세포이식입소서액구피하6주후칙가견기태류형성.결론:배태간세포원성간간세포이식입치료성간재생모형소서간내후가유효재간내능진일보생장분화병부분표체간세포공능;차차이식안전성교호.
    BACKGROUND: In vitro differentiation of embryonic stem cells into hepatocytes has been successfully reported to a certain degree; however, whether embryonic stem cells are able to effectively enter hepatic plate of host after intrahepatic transplantation, whether embryonic stem cells can further differentiate into hepatocytes and express hepatocyte function, and risk factors for neoplastic formation are still unclear at present. OBJECTIVE: To study the intrahepatic transplantation of embryonic stem cells-derived hepatic stem cells in therapeutic liver repopulation models, and to investigate the liver tissue replacement, growth and differentiation in vivo, and neoplastic formation.DESIGN: Randomized controlled animal study.SETTING: Department of Pediatric Surgery, the Second Hospital affiliated to Sun Yat-sen University. MATERIALS: Twenty-four BALB/c mice, 6-8 weeks old, weighing 20-35 g, irrespective of gender, were provided by Guangzhou Experimental Animal Center. Embryonic stem cells-derived hepatic stem cells were differentiated from embryonic stem cells. E14 was provided by Stem cell Center of our hospital. METHODS: This study was performed at the Stem Cell Center, the Second Hospital affiliated to Sun Yat-sen University from July 2006 to June 2007. Twenty-four mice were randomly divided into a liver repopulation model + stem cell transplantation group (group A) and a liver resection + stem cell transplantation group (group B), with 12 mice in each group. Mice in the group A were intraperitoneally injected with 50 mg/kg retrorsine once every two weeks for totally twice. Four weeks after the second injection, about 70% liver was resected. And then, the embryonic stem cells-derived hepatic stem cells, labeled by 1×105 carboxy fluoresce in diacetate succinimidyl ester (CFDA-SE), were transplanted into mouse liver through portal vein. On the other hand, 70% liver of mice in the group B was resected and embryonic stem cells-derived hepatic stem cells were transplanted into mouse liver. MAIN OUTCOME MEASURES: The distribution, incorporation, and proliferation of transplanted cells were observed under fluorescent microscopy. Two weeks later, hepatic function was stained with albumin fluorescence immunoassay (double fluorescence staining) and assayed by level of serum albumin. Embryonic stem cells-derived hepatic stem cells were poured into liver of remedial liver regeneration mice, and undifferentiated embryonic stem cells were transplanted into subcutaneous tissue in axillary region as the controls to observe neoplastic formation in embryonic stem cells-derived hepatic stem cells. RESULTS: ① Growth of hepatic stem cells in recipient mice: One week after transplantation of CFDA-SE-labeled embryonic stem cells-derived hepatic stem cells, some scattered region was green under fluorescent microscopy. The area of green region increased apparently in 2 weeks, and cord-like structure could be observed. ② Liver function: Immunofluorescent staining of albumin (double fluorescence staining) demonstrated that labeled cells expressed positive albumin (yellow fluorescence) in liver tissue of recipient mice, but there was not significant difference in serum albumin level between group A and group B (P > 0.05). ③ Reliability of hepatic stem cell transplantation: Teratoma did not form over 6 months; however, transplantation of undifferentiated embryonic stem cells in the axillary region could cause formation of teratoma after 6 weeks. CONCLUSION: The transplantation of embryonic stem cells-derived hepatic stem cells in therapeutic liver repopulation model mice can effectively and further grow and differentiate, or even partially express hepatocyte function; in particular, the transplantation is safe.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문