CAJ | 학술논문

目的:比较急性缺氧与缺氧合并双侧颈动脉结扎对新生猪缺氧缺血性脑损伤(HIBD)模型制备的影响.方法:生后7 d的新生猪24只,分成(1)缺氧组(H):呼吸机辅助通气吸入低浓度氧,(2)缺氧缺血组(HI):缺氧同时结扎双侧颈总动脉.两组新生猪分别吸入10%、8%和6%的低浓度氧30 min造成轻度、中度和重度缺氧,检测两组新生猪在不同程度缺氧下生理参数、脑血容量、脑氧灌注的变化.结果:H及HI组新生猪在不同程度缺氧后,两组间血气分析氧饱和度(SaO_2)、血乳酸浓度、血液pH值的差异无统计学意义(P＞0.05);重度缺氧时HI组新生猪的平均动脉压明显低于H组(P＜0.05).H及HI组新生猪在不同程度的缺氧后,脑血流量相对稳定,两组间脑血流改变的差异无统计学意义(P＞0.05);脑氧灌注明显下降,与缺氧前相比差异有统计学意义(P＜0.05),但两组问脑氧灌注改变的差异无统计学意义(P＞0.05).结论:H及HI这两种动物模型,对缺氧后生理参数、脑血容量和脑灌注的影响是基本一致的,呼吸机辅助吸入低浓度氧即可制备HIBD模型,可能不一定需要双侧颈动脉结扎.
목적:비교급성결양여결양합병쌍측경동맥결찰대신생저결양결혈성뇌손상(HIBD)모형제비적영향.방법:생후7 d적신생저24지,분성(1)결양조(H):호흡궤보조통기흡입저농도양,(2)결양결혈조(HI):결양동시결찰쌍측경총동맥.량조신생저분별흡입10%、8%화6%적저농도양30 min조성경도、중도화중도결양,검측량조신생저재불동정도결양하생리삼수、뇌혈용량、뇌양관주적변화.결과:H급HI조신생저재불동정도결양후,량조간혈기분석양포화도(SaO_2)、혈유산농도、혈액pH치적차이무통계학의의(P＞0.05);중도결양시HI조신생저적평균동맥압명현저우H조(P＜0.05).H급HI조신생저재불동정도적결양후,뇌혈류량상대은정,량조간뇌혈류개변적차이무통계학의의(P＞0.05);뇌양관주명현하강,여결양전상비차이유통계학의의(P＜0.05),단량조문뇌양관주개변적차이무통계학의의(P＞0.05).결론:H급HI저량충동물모형,대결양후생리삼수、뇌혈용량화뇌관주적영향시기본일치적,호흡궤보조흡입저농도양즉가제비HIBD모형,가능불일정수요쌍측경동맥결찰.
Objective:To compare the newborn piglet models of hypoxic ischemic brain damage in hypoxia and hypoxia combined with occlusion of both carotid arteries. Methods: Twenty four 7-day-old piglets were divided into two groups. Group H: mechanical ventilation with low concentration of oxygen,Group HI; mechanical ventilation with low concentration of oxygen combined with occlusion of both carotid arteries. The piglets were inhaled with 10%, 8%, and 6% low-concentration oxygen for 30 min, and grouped into mild, moderate, and severe hypoxia ones. The changes of physiological parameter, cerebral blood flow and cerebral oxygen perfusion were detected. Results: There were no significant differences in blood gas analysis of oxygen saturation, blood lactic acid and pH between the two groups(P＞0. 05) . The mean arterial pressure of severe hypoxia in HI was significantly lower than in H(P＜0.05). The cerebral blood flow in H and HI was relatively stable after different degrees of hypoxia. As compared with the cerebral blood flow perfusion in group H and HI, there were no significant differences between them ( P>0. 05). The cerebral oxygen perfusion in H and HI was significantly descent after different degrees of hypoxia (P＜ 0.05). As compared with the cerebral oxygen perfusion in groups H and HI, there were no significant differences between them. Conclusion: H and HI have the same effect on physiological parameter, cerebral volume and cerebral oxygen perfusion of newborn piglets. The mechanical ventilation with low concentration of oxygen to newborn piglets can develop the HIBD model, it is not necessary to occlude carotid arteries.