CAJ | 학술논문

通过研究不同温度和光照时间条件下双齿围沙蚕Perinereis aibuhitensis卵细胞的生长状况,分析了沙蚕卵细胞发育与环境因子的关系.结果表明:对于卵黄合成期的双齿围沙蚕,升温和延长光照时间可以加速卵细胞生长.在直接升温且光照周期为L: D=16: 8和L: D=8: 16的条件下,沙蚕卵细胞的生长速度分别为(2.46±0.06)×10~(-2)、(2.26±0.1)×10~(-2)μm~3/d;在经历低温后再升温且光照周期为L: D=16: 8和L: D=8: 16的条件下,沙蚕卵细胞的生长速度分别为(3.21±0.09)×10~(-2)、(2.56±0.05)×10~(-2)μm~3/d.双因素方差分析结果显示:恒定低温对卵黄合成期的沙蚕卵细胞快速生长有促进作用,其与未经历低温的沙蚕卵细胞生长速度比较差异极显著(P<0.01);光照对卵细胞生长速度的影响差异极显著(P<0.01);低温和光照的交互作用对沙蚕卵细胞的生长速度影响差异极显著(P<0.01).另外,低温对沙蚕卵径变异系数影响极显著(P<0.01),光照对沙蚕卵细胞变异系数影响不显著(P>0.05),低温和光照对卵细胞变异系数的交互影响不显著(P>0.05).
통과연구불동온도화광조시간조건하쌍치위사잠Perinereis aibuhitensis란세포적생장상황,분석료사잠란세포발육여배경인자적관계.결과표명:대우란황합성기적쌍치위사잠,승온화연장광조시간가이가속란세포생장.재직접승온차광조주기위L: D=16: 8화L: D=8: 16적조건하,사잠란세포적생장속도분별위(2.46±0.06)×10~(-2)、(2.26±0.1)×10~(-2)μm~3/d;재경력저온후재승온차광조주기위L: D=16: 8화L: D=8: 16적조건하,사잠란세포적생장속도분별위(3.21±0.09)×10~(-2)、(2.56±0.05)×10~(-2)μm~3/d.쌍인소방차분석결과현시:항정저온대란황합성기적사잠란세포쾌속생장유촉진작용,기여미경력저온적사잠란세포생장속도비교차이겁현저(P<0.01);광조대란세포생장속도적영향차이겁현저(P<0.01);저온화광조적교호작용대사잠란세포적생장속도영향차이겁현저(P<0.01).령외,저온대사잠란경변이계수영향겁현저(P<0.01),광조대사잠란세포변이계수영향불현저(P>0.05),저온화광조대란세포변이계수적교호영향불현저(P>0.05).
The growth of germ cells in sludge worm Perinereis aibuhitensis was determined at various temperature and photoperiods. The growth of the germ cells in the direct warming group was found to be (2.46±0.06)×10~(-2)μm~3/d and (2.26±0.1)×10~(-2)μm~3/d at L: D=16: 8 and L: D=8: 16, respectively. In the cooling group, however, the germ cells had growth rate of (3.21±0.09)×10~(-2)μm~3/d and (2.56±0.05)×10~(-2)μm~3/d at L: D=16: 8 and L: D=8: 16, respectively. Two factor analysis of variance indicated that the difference in growth of germ cells in stages of vitellogenesis in constant low temperature was very significant(P<0.01),and that the difference in photoperiod was significant (P<0.01). The effect of growth of germ cells on the interaction between low temperature and photoperiod was very significant (P<0.01). In addition, the effect of the low temperature on variation in diameters of germ cells was very significant (P<0.01).The effect of the photoperiod on diameters of germ cells was not significant (P>0.05), and the effect of the interaction between low temperature and photoperiod on variation in growth of germ cells was not significant (P>0.05).s very sign