CAJ | 학술논문

为研究~(60)Co-γ射线对榆黄菇菌丝遗传诱变的影响,采用不同的辐照剂量和不同的辐照剂量率对出发菌株榆黄菇818的尖端菌丝进行辐照处理.结果表明:在辐照剂量为600Gy、辐照剂量率为43.5Gy/h的条件下,菌丝再生后进行挑选,获得了生长优良的诱变菌株.将诱变菌株与出发菌株进行生长对比实验,诱变菌株菌丝生长速度平均提高21.1%;经拮抗实验、酯酶和过氧化物酶同工酶实验,证明诱变菌株遗传物质发生了变化,已不同于出发菌株,具有种的特异性;经连续转接6代进行固体培养,诱变菌株优良的生长性能得到了保持,具有遗传稳定性.
위연구~(60)Co-γ사선대유황고균사유전유변적영향,채용불동적복조제량화불동적복조제량솔대출발균주유황고818적첨단균사진행복조처리.결과표명:재복조제량위600Gy、복조제량솔위43.5Gy/h적조건하,균사재생후진행도선,획득료생장우량적유변균주.장유변균주여출발균주진행생장대비실험,유변균주균사생장속도평균제고21.1%;경길항실험、지매화과양화물매동공매실험,증명유변균주유전물질발생료변화,이불동우출발균주,구유충적특이성;경련속전접6대진행고체배양,유변균주우량적생장성능득도료보지,구유유전은정성.
The effect of ~(60)Co-γirradiation with different doses and dose rates on mycelial growth of Pleurotus citrinopileatus was investigated in this study. Mutant swains of Pleurotus citrinopileatus with high growth rate were obtained through~(60)Co-γ irradiation at 600 Gy and 43.5 Gy/h dose rate. After the regeneration of mycelia, a novel strain having the highest growth rate was screened, which showed an average increase of 21.1% when compared with that of the parental strain. Results of antagonistic test, esterase isozyme and peroxidase isozyme electrophoresis revealed significant genetic differences between the mutant strain and its parent stain. After six generations of continuous cultivation, the mutant strain kept the good growth vigor, thereby displaying high genetic stability.