CAJ | 학술논문

目的:建立一种反相高效液相色谱(RP-HPLC)检测法,用于测定大鼠脑组织中吗啡-3-葡萄糖醛酸苷(M3G)的浓度.方法:微粒体酶体外反应体系,经孵育和处理后,采用HPLC分析定量,并进行方法学考察.RP-HPLC测定采用Diamonsil ~(TM)C_(18)色谱柱(250 mm×4.6 mm,5 μm),流动相为10 mmol·L~(-1) 磷酸二氢钾缓冲液(含1 mmol·L~(-1)十二烷基磺酸钠,磷酸调pH=2.15):乙腈(79.5∶20.5),流速:1.5 ml·min~(-1),紫外检测波长:210 nm,进样量50 μl.结果:本实验条件下各物质分离良好,M3G线性范围50-600 μg· L~(-1). M3G最低检测限为50 μg· L~(-1).M3G的方法回收率在90%-105%范围内,提取回收率在75%以上.结论:本法可用于对大鼠脑微粒体体外孵育生成吗啡-3-葡萄糖醛酸苷含量的测定.
목적:건립일충반상고효액상색보(RP-HPLC)검측법,용우측정대서뇌조직중마배-3-포도당철산감(M3G)적농도.방법:미립체매체외반응체계,경부육화처리후,채용HPLC분석정량,병진행방법학고찰.RP-HPLC측정채용Diamonsil ~(TM)C_(18)색보주(250 mm×4.6 mm,5 μm),류동상위10 mmol·L~(-1) 린산이경갑완충액(함1 mmol·L~(-1)십이완기광산납,린산조pH=2.15):을정(79.5∶20.5),류속:1.5 ml·min~(-1),자외검측파장:210 nm,진양량50 μl.결과:본실험조건하각물질분리량호,M3G선성범위50-600 μg· L~(-1). M3G최저검측한위50 μg· L~(-1).M3G적방법회수솔재90%-105%범위내,제취회수솔재75%이상.결론:본법가용우대대서뇌미립체체외부육생성마배-3-포도당철산감함량적측정.
Objective:To establish a reversed-phase high performance liquid chromatography(RP-HPLC)method for the determination of morphine's metabolite-M3G in rat brain. Methods:Chromatographic separation was achieved with a Diamonsil~(TM)C_(18) analytical column(250 mm× 4.6 mm, 5 μm). The mobile phase was 10 mmol·L~(-1) potassium phosphate and 1 mmol ·L~(-1) sodium dodecyl sulfate(pH=2.15 adjusted by phosphoric acid)- acetonitrile(79.5∶20.5),and flow rate was 1.5 ml · min~(-1). The drug concentrations were measured by UV at 210 nm. Results:The calibration curves were linear in the concentration range of 50-600 μg · L~(-1) for M3G. The limit of quantitation(LOQ)was 50 μg· L~(-1) for M3G. The method recovery of the sample was within 90%-105%,the absolute recovery was above 75%. Conclusion:This method is simple and rapid,especially for quantitative analysis of M3G in rat brain in vitro.