CAJ | 학술논문

目的:探讨Rho GTPases在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及其临床意义.方法:应用RT-PCR和免疫组织化学法检测36例NSCLC组织Rho GTPases信号转导途径中的RhoC、E-钙黏附素、基质金属蛋白酶-2(matrix metalloproteinases-2, MMP-2)和MMP-9的表达.通过绘制Kaplan-Meier生存曲线分析RhoC mRNA的表达与患者预后的关系.结果: RhoC mRNA在NSCLC组织和癌旁正常组织中的表达存在差异(P<0.01),RhoC mRNA的表达与患者的性别、年龄、肿瘤浸润程度、淋巴结转移、肿瘤大小、组织学类型和分化程度无关,而与不同TNM分期有关(P<0.05).RhoC蛋白与MMP-2蛋白的表达呈正相关(r=0.474,P=0.003).RhoC mRNA低表达组患者的生存时间长于高表达组患者,但2者之间差异无统计学意义. 结论:RhoC mRNA的高表达可能与NSCLC的发生和发展有关,也可能与NSCLC的早期及中期浸润和转移有关.
목적:탐토Rho GTPases재비소세포폐암(non-small cell lung cancer,NSCLC)중적표체급기림상의의.방법:응용RT-PCR화면역조직화학법검측36례NSCLC조직Rho GTPases신호전도도경중적RhoC、E-개점부소、기질금속단백매-2(matrix metalloproteinases-2, MMP-2)화MMP-9적표체.통과회제Kaplan-Meier생존곡선분석RhoC mRNA적표체여환자예후적관계.결과: RhoC mRNA재NSCLC조직화암방정상조직중적표체존재차이(P<0.01),RhoC mRNA적표체여환자적성별、년령、종류침윤정도、림파결전이、종류대소、조직학류형화분화정도무관,이여불동TNM분기유관(P<0.05).RhoC단백여MMP-2단백적표체정정상관(r=0.474,P=0.003).RhoC mRNA저표체조환자적생존시간장우고표체조환자,단2자지간차이무통계학의의. 결론:RhoC mRNA적고표체가능여NSCLC적발생화발전유관,야가능여NSCLC적조기급중기침윤화전이유관.
Objective:To investigate the expression of Rho GTPases signaling pathway in non-small cell lung carcinoma (NSCLC)and its clinical significance.Methods:Molecules of Rho GTPases signaling pathway including RhoC, E-cadherin, MMP-2, and MMP-9 were detected by RT-PCR and immunohistochemistry in 36 specimens of non-small cell lung carcinoma (NSCLC). The relationship between RhoC mRNA and prognosis of patients was evaluated by using Kaplan-Meier survival curve analysis.Results:There was a significant difference in the expression of RhoC mRNA between NSCLC tissues and para-cancerous tissues (P<0.01). The expression of RhoC mRNA in NSCLC was not correlated with gender, age, invasion degree, lymph node metastasis, tumor size, histological classification, and differentiation degree but correlated with different TNM stage (P<0.05). There was a significant positive correlation between the expression of RhoC protein and MMP-2 protein (r=0.474, P=0.003). The survival time of patients with weak expression of RhoC mRNA was longer than those with over-expression of RhoC mRNA, but there was no significant difference between the two groups (P>0.05). Conclusion:Over-expression of RhoC mRNA is closely correlated with the tumorigenesis and progression of NSCLC, and may be related with invasion and metastasis of NSCLC early to middle stage.