浙江大学学报（农业与生命科学版）
浙江大學學報（農業與生命科學版）
절강대학학보（농업여생명과학판）
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2012年
1期
1-9
,共9页
水稻%高温%活性氧%胁迫%耐热性
水稻%高溫%活性氧%脅迫%耐熱性
수도%고온%활성양%협박%내열성
rice%high temperature%reactive oxygen species%stress%thermo-tolerance
对来自不同国家和地区的72个水稻品种在抽穗扬花期进行高温处理(40℃/35℃,14 h/10 h,12 d),记录结实率,分析和耐热相关的生理学特征,并鉴定耐热水稻的基因型.2年的试验结果表明:高温对结实率有严重的伤害,且基因型间存在明显的差异；苗期高温处理显著降低了叶绿素含量( SPAD值)、株高、根长和生物量,且对敏感基因型的影响较耐热基因型大；同时,高温胁迫导致膜脂过氧化,表现为丙二醛含量增加和超氧化物歧化酶活性增强；与敏感基因型相比,耐热基因型的丙二醛含量增加较少,而超氧化物歧化酶活性增加较多；根据试验结果,春江06、密阳46和京系17为高耐热基因型,明恢63、Kasalath和IRAT4217为高热敏感基因型.
對來自不同國傢和地區的72箇水稻品種在抽穗颺花期進行高溫處理(40℃/35℃,14 h/10 h,12 d),記錄結實率,分析和耐熱相關的生理學特徵,併鑒定耐熱水稻的基因型.2年的試驗結果錶明:高溫對結實率有嚴重的傷害,且基因型間存在明顯的差異；苗期高溫處理顯著降低瞭葉綠素含量( SPAD值)、株高、根長和生物量,且對敏感基因型的影響較耐熱基因型大；同時,高溫脅迫導緻膜脂過氧化,錶現為丙二醛含量增加和超氧化物歧化酶活性增彊；與敏感基因型相比,耐熱基因型的丙二醛含量增加較少,而超氧化物歧化酶活性增加較多；根據試驗結果,春江06、密暘46和京繫17為高耐熱基因型,明恢63、Kasalath和IRAT4217為高熱敏感基因型.
대래자불동국가화지구적72개수도품충재추수양화기진행고온처리(40℃/35℃,14 h/10 h,12 d),기록결실솔,분석화내열상관적생이학특정,병감정내열수도적기인형.2년적시험결과표명:고온대결실솔유엄중적상해,차기인형간존재명현적차이；묘기고온처리현저강저료협록소함량( SPAD치)、주고、근장화생물량,차대민감기인형적영향교내열기인형대；동시,고온협박도치막지과양화,표현위병이철함량증가화초양화물기화매활성증강；여민감기인형상비,내열기인형적병이철함량증가교소,이초양화물기화매활성증가교다；근거시험결과,춘강06、밀양46화경계17위고내열기인형,명회63、Kasalath화IRAT4217위고열민감기인형.
The experiments were conducted to identify thermo-tolerant rice genotypes and characterize physiological traits relevant to heat stress tolerance.Seventy-two rice genotypes were exposed to high temperature (40 ℃/35 ℃,14 h/10 h) for 12 d at heading stage,and floret fertility was examined.The similar results were obtained in the two years' experiments that there was a distinctly genotypic difference in floret fertility as affected by heat stress.When rice seedlings were exposed to heat stress,chlorophyll content (SPAD value),plant height,root length,shoot and root biomass were dramatically reduced,with thermo-tolerant genotypes being relatively less affected than sensitive ones.Meanwhile,high temperature enhanced membrane lipid peroxidation,as expressed by malondialdehyde content and increased superoxide dismutase (SOD) activity.Thermo-tolerant genotypes had less and more increase in MDA content and SOD activity than sensitive ones under heat stress relative to the control,respectively.Accordingly the thermo-tolerant genotypes were identified,with genotypes CJ6,MY46 and JX17 being highly thermo-tolerant,MH63,Kasalath and IRAT4217 being very sensitive.
