CAJ | 학술논문

目的基质金属蛋白酶9基因多态性与慢性阻塞性肺疾病(COPD)易感性的关系.方法应用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测69例COPD患者,61例健康者基质金属蛋白酶9 R279Q位点等位基因和基因型.结果 ①COPD患者基质金属蛋白酶9 R279Q位点突变型(QQ)及杂合型(R/Q)基因型频率为31.8％和14.6％,健康对照组分别为37.8％和26.2％,两组基因分布频率差异无统计学意义；而野生型(RR)在两组中分布频率分别为53.6％和36％,其分布频率差异有统计学意义(P＜0.05)；在COPD组和健康对照组中R和Q等位基因频率差异有统计学意义(P＜0.05).②在吸烟者中,QQ及R/Q基因型在两组中分布差异无统计学意义；而RR基因型在两组中分布差异有统计学意义(P＜0.05).不吸烟者中,QQ、R/Q、RR基因型在分布差异均无统计学意义.吸烟者中,R和Q等位基因频率在两组中分布差异有统计学意义(P＜0.05)；在不吸烟者中,R和Q等位基因频率在两组中分布差异无统计学意义.结论 ①MMP-9 R279Q基因多态性可能是COPD发病的危险因素.②R等位基因可能为吸烟者患COPD的一个易感因素.
목적 기질금속단백매9기인다태성여만성조새성폐질병(COPD)역감성적관계.방법 응용취합매련반응-한제성편단장도다태성법(PCR-RFLP)검측69례COPD환자,61례건강자기질금속단백매9 R279Q위점등위기인화기인형.결과 ①COPD환자기질금속단백매9 R279Q위점돌변형(QQ)급잡합형(R/Q)기인형빈솔위31.8％화14.6％,건강대조조분별위37.8％화26.2％,량조기인분포빈솔차이무통계학의의；이야생형(RR)재량조중분포빈솔분별위53.6％화36％,기분포빈솔차이유통계학의의(P＜0.05)；재COPD조화건강대조조중R화Q등위기인빈솔차이유통계학의의(P＜0.05).②재흡연자중,QQ급R/Q기인형재량조중분포차이무통계학의의；이RR기인형재량조중분포차이유통계학의의(P＜0.05).불흡연자중,QQ、R/Q、RR기인형재분포차이균무통계학의의.흡연자중,R화Q등위기인빈솔재량조중분포차이유통계학의의(P＜0.05)；재불흡연자중,R화Q등위기인빈솔재량조중분포차이무통계학의의.결론 ①MMP-9 R279Q기인다태성가능시COPD발병적위험인소.②R등위기인가능위흡연자환COPD적일개역감인소.
Objective To investigate the correlation between matrix metalloproteinase-9 (MMP-9)gene polymorphism and the susceptibility of chronic obstructive pulmonary disease(COPD).Methods Examine the frequency of the genetype of MMP-9 in 69 patients and 61 control subjects by reslriction fragment length polymorphism.Results ①The frequency of MMP-9 QQ,R/Q genotype were 31.8％,14.6％ in COPD group,respectively,and 37.8％,26.2％ in control group,respectively.There were no significant differences between the two groups ( P ＞0.05).The distribution frequencies of RR genotypes were significantly different between two groups ( P ＜0.05).The differences in allele frequencies were also significant between the two groups ( P ＜0.05).②Smokers,there were no significant differences on QQ,R/ Q genotypes in COPD group and in controls.There were significantly different on RR genotypes in COPD group and in controls ( P ＜0.05).In non-smokers,there were no significant differences on QQ,R/Q,RR genotypes in two group.Smokers,there were significantly different on the frequencies allele of R and Q between two groups.In non-smokers,there were no significant differences on the frequencies allele of R and Q between two groups.Conclusious ①MMP-9 R279Q polymorphisms maybe a genetic risk factor for COPD.②The allele gene of R maybe a susceptibility factor for COPD in smokers.